An electrochemical biosensor based on DNA tetrahedron/graphene composite film for highly sensitive detection of NADH

被引:36
作者
Li, Zonglin [1 ]
Su, Wenqiong [2 ]
Liu, Shuopeng [1 ]
Ding, Xianting [1 ]
机构
[1] Shanghai Jiao Tong Univ, Med X Res Inst, Shanghai 200240, Peoples R China
[2] Shanghai Jiao Tong Univ, State Key Lab Met Matrix Composities, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
基金
中国国家自然科学基金;
关键词
NADH detection; Electrochemical; Gold nanoparticles; DNA tetrahedron; Graphene; NICOTINAMIDE ADENINE-DINUCLEOTIDE; GOLD NANOPARTICLES; CATALYTIC-ACTIVITY; CARBON ELECTRODES; CELL-DEATH; GRAPHENE; OXIDATION; ORIGAMI; NANOSTRUCTURES; PLATFORM;
D O I
10.1016/j.bios.2015.02.031
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Dihydronicotinamide adenine dinucleotide (NADH) is a major biomarker correlated with lethal diseases such as cancers and bacterial infection. Herein, we report a graphene-DNA tetrahedron-gold nanoparticle modified gold disk electrode for highly sensitive NADH detection. By assembling the DNA tetrahedron/graphene composite film on the gold disk electrode surface which prior harnessed electrochemical deposition of gold nanoparticles to enhance the effective surface area, the oxidation potential of NADH was substantially decreased to 0.28 V (vs. Ag/AgCl) and surface fouling effects were successfully eliminated. Furthermore, the lower detection limit of NADH by the presented platform was reduced down to 1 fM, with an upper limit of 10 pM. Both the regeneration and selectivity of composite film-modified electrode are investigated and proved to be robust. The novel sensor developed here could serve as a highly sensitive probe for NADH detection, which would further benefit the field of NADH related disease diagnostics. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:287 / 293
页数:7
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