Use of loop-mediated isothermal amplification of DNA for the rapid detection of Mycobacterium tuberculosis in clinical specimens

被引：37

作者：

Neonakis, I. K. ^{[1
,2
]}

Spandidos, D. A. ^{[3
]}

Petinaki, E. ^{[2
]}

机构：

[1] Univ Hosp Herakl, Dept Microbiol, Iraklion 71201, Crete, Greece

[2] Univ Thessaly, Sch Med, Dept Microbiol, Larisa, Greece

[3] Univ Crete, Sch Med, Dept Lab Med, Iraklion, Greece

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2011年 / 30卷 / 08期

关键词：

SPUTUM SAMPLES; LAMP; MAGNESIUM; DIAGNOSIS; TEMPLATE; SEQUENCE; ASSAY;

D O I：

10.1007/s10096-011-1195-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Loop-mediated isothermal amplification (LAMP) is a recently developed molecular method that has been successfully implemented in the detection of Mycobacterium tuberculosis in clinical specimens. LAMP has several advantages, such as rapidity, high sensitivity, ease of application and cost-effectiveness. As a result, it is anticipated that its use for the detection of M. tuberculosis is likely to become widespread, especially in low-resource countries. The present review aimed to present this method and all of the available information on its implementation in the detection of M. tuberculosis in clinical specimens.

引用

页码：937 / 942

页数：6

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