RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA Test v1.5) for Early Diagnosis of HIV-1 Infection in Infants in Senegal

被引：17

作者：

Kebe, K. ^{[1
]}

Ndiaye, O. ^{[1
]}

Ndiaye, H. Diop ^{[1
]}

Mengue, P. Mbakob ^{[1
]}

Guindo, P. M. M. ^{[1
]}

Diallo, S. ^{[1
]}

Leye, N. ^{[1
]}

Gueye, S. B. ^{[1
]}

Diallo, A. Gaye ^{[1
]}

Kane, C. Toure ^{[1
]}

Mboup, S. ^{[1
]}

机构：

[1] Univ Cheikh Anta Diop, Lab Bacteriol Virol, Hop Aristide le Dantec, Dakar, Senegal

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 07期

关键词：

POLYMERASE-CHAIN-REACTION; DRIED BLOOD SPOTS; IMMUNODEFICIENCY-VIRUS-INFECTION; REAL-TIME PCR; RESOURCE-LIMITED SETTINGS; TYPE-1; INFECTION; WHOLE-BLOOD; ANTIGEN-DETECTION; P24; ANTIGEN; ASSAY;

D O I：

10.1128/JCM.02402-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMerieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.

引用

页码：2590 / 2593

页数：4

共 32 条

[1] RAPID SCREENING FOR EARLY DETECTION OF MOTHER-TO-CHILD TRANSMISSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

CASSOL, S ;

BUTCHER, A ;

KINARD, S ;

SPADORO, J ;

SY, T ;

LAPOINTE, N ;

READ, S ;

GOMEZ, P ;

FAUVEL, M ;

MAJOR, C ;

OSHAUGHNESSY, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (11) :2641-2645

[2]

CASSOL SA, 1992, J ACQ IMMUN DEF SYND, V5, P113

[3]

COMEAU AM, 1992, J ACQ IMMUN DEF SYND, V5, P271

[4] Infant human immunodeficiency virus diagnosis in resource-limited settings: issues, technologies, and country experiences [J].

Creek, Tracy L. ;

Sherman, Gayle G. ;

Nkengasong, John ;

Lu, Lydia ;

Finkbeiner, Thomas ;

Fowler, Mary Glenn ;

Rivadeneira, Emilia ;

Shaffer, Nathan .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2007, 197 (03) :S64-S71

[5] Comparison of human immunodeficiency virus 1 DNA polymerase chain reaction and qualitative and quantitative RNA polymerase chain reaction in human immunodeficiency virus 1-exposed infants [J].

Cunningham, CK ;

Charbonneau, TT ;

Song, KM ;

Patterson, D ;

Sullivan, T ;

Cummins, T ;

Poiesz, B .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1999, 18 (01) :30-35

[6]

DEROSSI A, 1991, AIDS, V5, P15, DOI 10.1097/00002030-199101000-00002

[7] DESIGN AND EVALUATION OF NEW, HIGHLY SENSITIVE AND SPECIFIC PRIMERS FOR POLYMERASE CHAIN-REACTION DETECTION OF HIV-1-INFECTED PRIMARY LYMPHOCYTES [J].

FRANSEN, K ;

ZHONG, P ;

DEBEENHOUWER, H ;

CARPELS, G ;

PEETERS, M ;

LOUWAGIE, J ;

JANSSENS, W ;

PIOT, P ;

VANDERGROEN, G .

MOLECULAR AND CELLULAR PROBES, 1994, 8 (04) :317-322

[8] Quantitation of HIV-1 RNA in dried blood spots by the real-time NucliSENS EasyQ HIV-1 assay in Senegal [J].

Kane, Coumba Toure ;

Ndiaye, Halimatou Diop ;

Diallo, Sada ;

Ndiaye, Ibrahima ;

Wade, Abdoulaye Sidibe ;

Diaw, Papa Alassane ;

Gaye-Diallo, Aissatou ;

Mboup, Souleymane .

JOURNAL OF VIROLOGICAL METHODS, 2008, 148 (1-2) :291-295

[9] Dried blood spots for the diagnosis and quantitation of HIV-1: Stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand [J].

Leelawiwat, W. ;

Young, N. L. ;

Chaowanachan, T. ;

Ou, C. Y. ;

Culnane, M. ;

Vanprapa, N. ;

Waranawat, N. ;

Wasinrapee, P. ;

Mock, P. A. ;

Tappero, J. ;

McNicholl, J. M. .

JOURNAL OF VIROLOGICAL METHODS, 2009, 155 (02) :109-117

[10] Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots [J].

Lilian, R. R. ;

Bhowan, K. ;

Sherman, G. G. .

JOURNAL OF CLINICAL VIROLOGY, 2010, 48 (01) :40-43

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