Advancing formaldehyde cross-linking towards quantitative proteomic applications

被引：48

作者：

Klockenbusch, Cordula ^{[1
]}

O'Hara, Jane E. ^{[1
]}

Kast, Juergen ^{[1
,2
,3
]}

机构：

[1] Univ British Columbia, Biomed Res Ctr, Vancouver, BC V6T 1Z3, Canada

[2] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z3, Canada

[3] Univ British Columbia, Ctr Blood Res, Vancouver, BC V6T 1Z3, Canada

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2012年 / 404卷 / 04期

关键词：

FFPE; Formaldehyde cross-linking; Protein-protein interactions; Mass spectrometry; PARAFFIN-EMBEDDED TISSUES; PROTEIN-PROTEIN INTERACTIONS; IMAGING MASS-SPECTROMETRY; GENOME-WIDE ANALYSIS; IN-VIVO; AFFINITY PURIFICATION; ENERGY TRANSDUCTION; DNA INTERACTIONS; BINDING-SITES; IDENTIFICATION;

D O I：

10.1007/s00216-012-6065-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Formaldehyde is a key fixation reagent. This review explores its application in combination with qualitative and quantitative mass spectrometry (MS). Formalin-fixed and paraffin-embedded (FFPE) tissues form a large reservoir of biologically valuable samples and their investigation by MS has only recently started. Furthermore, formaldehyde can be used to stabilise protein-protein interactions in living cells. Because formaldehyde is able to modify proteins, performing MS analysis on these samples can pose a challenge. Here we discuss the chemistry of formaldehyde cross-linking, describe the problems of and progress in these two applications and their common aspects, and evaluate the potential of these methods for the future.

引用

页码：1057 / 1067

页数：11

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