Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance

被引：51

作者：

Li, Yuanyuan ^{[1
,2
,3
]}

Na, Rongwei ^{[1
,2
]}

Wang, Xiumei ^{[1
]}

Liu, Huiying ^{[2
]}

Zhao, Lingyun ^{[1
]}

Sun, Xiaodan ^{[1
]}

Ma, Guowu ^{[2
]}

Cui, Fuzhai ^{[1
]}

机构：

[1] Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Beijing 100084, Peoples R China

[2] Dalian Med Univ, Sch Stomatol, Dept Prosthodont, Dalian 116044, Peoples R China

[3] Shengli Oil Field Cent Hosp, Dept Stomatol, Dongying 257034, Peoples R China

来源：

MOLECULES | 2017年 / 22卷 / 10期

基金：

中国国家自然科学基金;

关键词：

antimicrobial peptides; electrospraying; bacterial resistance; PLGA; compositemicrospheres; DECAPEPTIDE KSL; NANOPARTICLES; DELIVERY; DRUG; IDENTIFICATION; ELECTROSPRAY;

D O I：

10.3390/molecules22101637

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH2), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment. In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods. Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity. Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria. Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1. Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.

引用

页数：12

共 31 条

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Jirwankar, Yugandhara B.
Dighe, Vikas D.
Jindal, Anil B.
MOLECULAR PHARMACEUTICS, 2020, 17 (10) : 3990 - 4003

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