Sulfated fucoidan FP08S2 inhibits lung cancer cell growth in vivo by disrupting angiogenesis via targeting VEGFR2/VEGF and blocking VEGFR2/Erk/VEGF signaling

被引：97

作者：

Chen, Huanjun ^{[1
,2
,3
]}

Cong, Qifei ^{[1
,2
,3
]}

Du, Zhenyun ^{[1
,2
,3
]}

Liao, Wenfeng ^{[1
,2
,3
]}

Zhang, Lei ^{[1
,2
,3
]}

Yao, Yanli ^{[1
,2
,3
]}

Ding, Kan ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China

[3] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

来源：

CANCER LETTERS | 2016年 / 382卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Fucoidan; Angiogenesis; VEGF; Lung cancer; TUMOR ANGIOGENESIS; VEGF; MECHANISMS; RECEPTOR; TRANSDUCTION; EXPRESSION; TISSUE; MODEL; GENE; KDR;

D O I：

10.1016/j.canlet.2016.08.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fucoidan may inhibit angiogenesis. However, its functional target molecule and the underlying mechanism are still vague. In the present study, we showed that sulfated fucoidan FP08S2 from Sargassum fusiforme inhibited tube formation as well as migration and invasion of human microvascular endothelial cells (HMEC-1). In addition, FP08S2 was confirmed to disrupt VEGF-induced angiogenesis both in vitro and in vivo. Further study indicated that FP08S2 could bind to both VEGF and VEGFR2 to interfere with VEGF-VEGFR2 interaction. Moreover, VEGFR2/Erk/VEGF signaling pathway was blocked by FP08S2 in HMEC-1 cells. Importantly, FP08S2 impeded the growth and microvessel formation of A549 cancer cell xenograft in nude mice. These results suggested that FP08S2 presented remarkable anti-angiogenic activity via blocking VEGF signaling and could be a potential novel leading compound to inhibit lung cancer cell growth. (C) 2016 Published by Elsevier Ireland Ltd.

引用

页码：44 / 52

页数：9

共 40 条

[1] Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds [J].