Molecular cloning and characterization of hagfish estrogen receptors

被引:6
|
作者
Nishimiya, Osamu [1 ]
Katsu, Yoshinao [2 ]
Inagawa, Hiroyuki [3 ]
Hiramatsu, Naoshi [1 ]
Todo, Takashi [1 ]
Hara, Akihiko [1 ]
机构
[1] Hokkaido Univ, Fac Fisheries Sci, Div Marine Life Sci, Lab Fish Reprod Physiol & Biochem, Hakodate, Hokkaido 0418611, Japan
[2] Hokkaido Univ, Fac Sci, Dept Biol Sci, Lab Reprod & Dev Biol, Sapporo, Hokkaido 0600810, Japan
[3] Natl Fisheries Univ, Dept Appl Aquabiol, Shimonoseki, Yamaguchi 7596595, Japan
来源
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2017年 / 165卷
关键词
Agnathan; Eptatretus burgeri; Estrogen receptor; Molecular cloning; Transactivation; ALPHA ER-ALPHA; TRANSCRIPTIONAL ACTIVATION; TISSUE DISTRIBUTION; CDNA CLONING; EXPRESSION; ANTAGONISM; GENERATION; AMPHIOXUS; SEQUENCE; STEROIDS;
D O I
10.1016/j.jsbmb.2016.06.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
One or more distinct forms of the nuclear estrogen receptor (ER) have been isolated from many vertebrates to date. To better understand the molecular evolution of ERs, we cloned and characterized er cDNAs from the inshore hagfish, Eptatretus burgeri, a modern representative of the most primitive vertebrates, the agnathans. Two er cDNAs, er1 and er2, were isolated from the liver of a reproductive female hagfish. A phylogenetic analysis placed hagfish ER1 into a position prior to the divergence of vertebrate ERs. Conversely, hagfish ER2 was placed at the base of the vertebrate ER beta Glade. The tissue distribution patterns of both ER subtype mRNAs appeared to be different, suggesting that each subtype has different physiological roles associated with estrogen actions. An estrogen responsive-luciferase reporter assay using mammalian HEK293 cells was used to functionally characterize these hagfish ERs. Both ER proteins displayed estrogen-dependent activation of transcription. These results clearly demonstrate that the hagfish has two functional ER subtypes. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:190 / 201
页数:12
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