Enzymology of the anaphase-promoting complex

被引：26
作者：
Carroll, CW [1 ]
Morgan, DO [1 ]
机构：
[1] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA
来源：
UBIQUITIN AND PROTEIN DEGRADATION, PART A | 2005年 / 398卷
关键词：
D O I：
10.1016/S0076-6879(05)98018-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC.
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收藏
页码：219 / 230
页数：12
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