Chromosomal organization of the 18S and 5S rRNAs and histone H3 genes in Scarabaeinae coleopterans: insights into the evolutionary dynamics of multigene families and heterochromatin

被引:66
作者
Cabral-de-Mello, Diogo C. [1 ]
Oliveira, Sarah G. [2 ]
de Moura, Rita C. [3 ]
Martins, Cesar [2 ]
机构
[1] UNESP Univ Estadual Paulista, Inst Biociencias IB, Dept Biol, Sao Paulo, Brazil
[2] UNESP Univ Estadual Paulista, Inst Biociencias IB, Dept Morfol, Sao Paulo, Brazil
[3] UPE Univ Pernambuco, Inst Ciencias Biol ICB, Dept Biol, Recife, PE, Brazil
基金
巴西圣保罗研究基金会;
关键词
TANDEMLY REPEATED UNIT; REPETITIVE DNA; TRANSPOSABLE ELEMENTS; REGIONS NORS; RDNA; BEETLE; LOCATION; SEQUENCE; CLUSTERS; REARRANGEMENTS;
D O I
10.1186/1471-2156-12-88
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background: Scarabaeinae beetles show a high level of macro-chromosomal variability, although the karyotypic organization of heterochromatin and multigene families (rDNAs and histone genes) is poorly understood in this group. To better understand the chromosomal organization and evolution in this group, we analyzed the karyotypes, heterochromatin distribution and chromosomal locations of the rRNAs and histone H3 genes in beetles belonging to eight tribes from the Scarabaeinae subfamily (Coleoptera, Scarabaeidae). Results: The number of 18S rRNA gene (a member of the 45S rDNA unit) sites varied from one to 16 and were located on the autosomes, sex chromosomes or both, although two clusters were most common. Comparison of the 45S rDNA cluster number and the diploid numbers revealed a low correlation value. However, a comparison between the number of 45S rDNA sites per genome and the quantity of heterochromatin revealed (i) species presenting heterochromatin restricted to the centromeric/pericentromeric region that contained few rDNA sites and (ii) species with a high quantity of heterochromatin and a higher number of rDNA sites. In contrast to the high variability for heterochromatin and 45S rDNA cluster, the presence of two clusters (one bivalent cluster) co-located on autosomal chromosomes with the 5S rRNA and histone H3 genes was highly conserved. Conclusions: Our results indicate that the variability of the 45S rDNA chromosomal clusters is not associated with macro-chromosomal rearrangements but are instead related to the spread of heterochromatin. The data obtained also indicate that both heterochromatin and the 45S rDNA loci could be constrained by similar evolutionary forces regulating spreading in the distinct Scarabaeinae subfamily lineages. For the 5S rRNA and the histone H3 genes, a similar chromosomal organization could be attributed to their association/co-localization in the Scarabaeinae karyotypes. These data provide evidence that different evolutionary forces act at the heterochromatin and the 45S rDNA loci compared to the 5S rRNA and histone H3 genes during the evolution of the Scarabainae karyotypes.
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页数:12
相关论文
共 63 条
[1]   INTERSPERSION OF HISTONE AND 5S RNA GENES IN ARTEMIA [J].
ANDREWS, MT ;
VAUGHN, JC ;
PERRY, BA ;
BAGSHAW, JC .
GENE, 1987, 51 (01) :61-67
[2]   Organization, nucleotide sequence, and chromosomal mapping of a tandemly repeated unit containing the four core histone genes and a 5S rRNA gene in an isopod crustacean species [J].
Barzotti, R ;
Pelliccia, F ;
Bucciarelli, E ;
Rocchi, A .
GENOME, 2000, 43 (02) :341-345
[3]   Genetics -: Junk DNA as an evolutionary force [J].
Biemont, Christian ;
Vieira, Cristina .
NATURE, 2006, 443 (7111) :521-524
[4]   A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3, staining and FISH techniques [J].
Bione, E ;
Camparoto, ML ;
Simoes, ZLP .
GENETICS AND MOLECULAR BIOLOGY, 2005, 28 (01) :111-116
[5]  
Bione E, 2005, GEN MOL BIOL, V26, P376
[6]   Cytogenetic Mapping of rRNAs and Histone H3 Genes in 14 Species of Dichotomius ( Coleoptera, Scarabaeidae, Scarabaeinae) Beetles [J].
Cabral-de-Mello, D. C. ;
Moura, R. C. ;
Martins, C. .
CYTOGENETIC AND GENOME RESEARCH, 2011, 134 (02) :127-135
[7]   Cytogenetic Mapping of 5S and 18S rRNAs and H3 Histone Genes in 4 Ancient Proscopiidae Grasshopper Species: Contribution to Understanding the Evolutionary Dynamics of Multigene Families [J].
Cabral-de-Mello, D. C. ;
Martins, C. ;
Souza, M. J. ;
Moura, R. C. .
CYTOGENETIC AND GENOME RESEARCH, 2011, 132 (1-2) :89-93
[8]   Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement [J].
Cabral-de-Mello, D. C. ;
Moura, R. C. ;
Martins, C. .
HEREDITY, 2010, 104 (04) :393-400
[9]   Cytogenetic analysis of two related Deltochilum (Coleoptera, Scarabaeidae) species: Diploid number reduction, extensive heterochromatin addition and differentiation [J].
Cabral-de-Mello, D. C. ;
Moura, R. C. ;
Carvalho, R. ;
Souza, M. J. .
MICRON, 2010, 41 (02) :112-117
[10]   Evolutionary dynamics of 5S rDNA location in acridid grasshoppers and its relationship with H3 histone gene and 45S rDNA location [J].
Cabral-de-Mello, Diogo C. ;
Cabrero, Josefa ;
Dolores Lopez-Leon, Maria ;
Camacho, Juan Pedro M. .
GENETICA, 2011, 139 (07) :921-931