The role of thiol redox systems in the resistance of Escherichia coli in the stationary phase

被引：1

作者：

Smirnova, G. V. ^{[1
]}

Muzyka, N. G. ^{[1
]}

Oktyabr'skii, O. N. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Ural Branch, Inst Ecol & Genet Microorganisms, Perm 614081, Russia

[2] Perm State Tech Univ, Perm 614990, Russia

来源：

MICROBIOLOGY | 2011年 / 80卷 / 05期

关键词：

starvation stress; glutathione; thioredoxin; gene expression; antibiotic resistance; SIGMA-FACTOR SIGMA(S); HYDROGEN-PEROXIDE; THIOREDOXIN; CELL; PATHWAYS; DEFENSE; DEATH;

D O I：

10.1134/S0026261711050146

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effect of glutathione (gshA), thioredoxin (trxA), and thioredoxin reductase (trxB) mutations on the adaptation of Escherichia coli to prolonged glucose starvation was investigated. The trxB mutation had the worst consequences for the stationary-phase cells. These bacteria exhibited decreased survival, increased sensitivity to oxidants, and decreased expression of the katE and sulA genes. As the stationary phase proceeded, the physiological resistance to antibiotics increased in all the strains tested; however, the thiol redox system mutants were far less able to develop antibiotic resistance than the parent strain cells. During the stationary phase, a considerable shift was observed in the redox status of intra- and extracellular glutathione toward the oxidative values. These results indicate that the thiol redox systems play an important role in the adaptation of Escherichia coli to prolonged starvation and antibiotic resistance.

引用

页码：631 / 637

页数：7

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