FUNCTIONAL CHARACTERIZATION OF HUMAN KINDLIN-2 CORE PROMOTER IDENTIFIES A KEY ROLE OF SP1 IN KINDLIN-2 TRANSCRIPTIONAL REGULATION

被引：1
作者：
Khan, Ammad Aslam [1 ]
Shimokawa, Takashi [1 ]
Stromblad, Staffan [1 ]
Zhang, Hongquan [1 ,2 ,3 ]
机构：
[1] Karolinska Inst, Dept Biosci & Nutr, Ctr Biosci, Novum, S-14183 Huddinge, Sweden
[2] Peking Univ, Sch Basic Med Sci, Lab Mol Cell Biol & Tumor Biol, Beijing 100191, Peoples R China
[3] Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100191, Peoples R China
来源：
CELLULAR & MOLECULAR BIOLOGY LETTERS | 2011年 / 16卷 / 04期
基金：
瑞典研究理事会;
关键词：
Kindlin-2; FERMT2; PLEKHC1; Mig-2; Transcription factor SP1; Promoter; Transcription start site; Gene regulation; Cis-acting elements; CpG island; Cell migration; Integrin; Gene expression; CELL-MATRIX ADHESION; INTEGRIN; LOCALIZATION; ACTIVATION; COMPONENT; UNC-112; PROTEIN; LENGTH; MOUSE;
D O I：
10.2478/s11658-011-0028-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located -122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.
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页码：638 / 651
页数：14
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