Anti-Inflammatory, Antioxidative, and Nitric Oxide-Scavenging Activities of a Quercetin Nanosuspension with Polyethylene Glycol in LPS-Induced RAW 264.7 Macrophages

被引:26
作者
Kang, Sang Gu [1 ]
Lee, Gi Baek [1 ]
Vinayagam, Ramachandran [1 ]
Do, Geum Sook [2 ]
Oh, Se Yong [3 ]
Yang, Su Jin [3 ]
Kwon, Jun Bum [3 ]
Singh, Mahendra [1 ]
机构
[1] Yeungnam Univ, Inst Biotechnol Life & Appl Sci, Dept Biotechnol, Gyongsan 38541, South Korea
[2] Kyungpook Natl Univ, Coll Nat Sci, Dept Biol, Daegu 41566, South Korea
[3] Nova M Healthcare Co Ltd, 16-53,Jisiksaneop 4-Ro, Gyongsan 38408, South Korea
来源
MOLECULES | 2022年 / 27卷 / 21期
基金
新加坡国家研究基金会;
关键词
quercetin; nanosuspension; antioxidant properties; flavonoids; LPS-induced inflammation; NO-scavenging activity; IN-VITRO; ALZHEIMERS-DISEASE; INFLAMMATION; INHIBITION; SOLUBILITY; EXTRACT; ENHANCEMENT; DISPERSIONS; DERIVATIVES; MECHANISMS;
D O I
10.3390/molecules27217432
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Quercetin (Qu) is a dietary antioxidant and a member of flavonoids in the plant polyphenol family. Qu has a high ability to scavenge reactive oxygen species (ROS) and reactive nitrogen species (RNS) molecules; hence, exhibiting beneficial effects in preventing obesity, diabetes, cancer, cardiovascular diseases, and inflammation. However, quercetin has low bioavailability due to poor water solubility, low absorption, and rapid excretion from the body. To address these issues, the usage of Qu nanosuspensions can improve physical stability, solubility, and pharmacokinetics. Therefore, we developed a Qu and polyethylene glycol nanosuspension (Qu-PEG NS) and confirmed its interaction by Fourier transform infrared analysis. Qu-PEG NS did not show cytotoxicity to HaCaT and RAW 264.7 cells. Furthermore, Qu-PEG NS effectively reduced the nitrogen oxide (NO) production in lipopolysaccharide (LPS)-induced inflammatory RAW 264.7 cells. Additionally, Qu-PEG NS effectively lowered the levels of COX-2, NF-kappa B p65, and IL-1 beta in the LPS-induced inflammatory RAW 264.7 cells. Specifically, Qu-PEG NS exhibited anti-inflammatory properties by scavenging the ROS and RNS and mediated the inhibition of NF-kappa B signaling pathways. In addition, Qu-PEG NS had a high antioxidant effect and antibacterial activity against Escherichia coli and Bacillus cereus. Therefore, the developed novel nanosuspension showed comparable antioxidant, anti-inflammatory, and antibacterial functions and may also improve solubility and physical stability compared to raw quercetin.
引用
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页数:21
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