Purification and characterization of a bacterial peroxidase from the isolated strain Pseudomonas sp SUK1 and its application for textile dye decolorization

被引：43

作者：

Kalyani, Dayanand C. ^{[1
]}

Phugare, Swapnil S. ^{[1
]}

Shedbalkar, Utkarsha U. ^{[1
]}

Jadhav, Jyoti P. ^{[1
]}

机构：

[1] Shivaji Univ, Dept Biochem, Kolhapur 416004, Maharashtra, India

来源：

ANNALS OF MICROBIOLOGY | 2011年 / 61卷 / 03期

关键词：

HPLC; L-DOPA; Methyl orange; Peroxidase; Pseudomonas sp; SUK1; SULFONATED AZO DYES; PHANEROCHAETE-CHRYSOSPORIUM; TRAMETES-VERSICOLOR; LIGNIN PEROXIDASE; BIODEGRADATION; DEGRADATION; LACCASE; RED; AZOREDUCTASE; STREPTOMYCES;

D O I：

10.1007/s13213-010-0162-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The intracellular bacterial peroxidase from Pseudomonas sp. SUK1 was purified by anion exchange and molecular sieve chromatography. The molecular weight of the purified peroxidase was estimated to be 86 kDa by SDS-PAGE analysis. The UV-visible absorption spectra revealed that the purified peroxidase was a heme-containing protein. The purified enzyme exerted its optimal activity with n-propanol and also oxidized various lignin-related phenols, whereas no activity was observed with p-cresol and veratrole. Optimum pH and temperature for the purified peroxidase were 3.0 and 40 degrees C, respectively. The purified enzyme proficiently decolorized various textile dyes. Methyl orange was used as a model azo dye to understand the mechanism of action of peroxidase in dye degradation. Further, dye degradation was confirmed by analytical techniques like FTIR, HPLC and GC-MS.

引用

页码：483 / 491

页数：9

共 37 条

[21]

Kim SJ, 1999, APPL ENVIRON MICROB, V65, P1029

[22] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[23]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[24] Microbial decolourisation and degradation of textile dyes [J].

McMullan, G ;

Meehan, C ;

Conneely, A ;

Kirby, N ;

Robinson, T ;

Nigam, P ;

Banat, IM ;

Marchant, R ;

Smyth, WE .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 56 (1-2) :81-87

[25] Purification and characterization of laccase produced by a white rot fungus Pleurotus sajor-caju under submerged culture condition and its potential in decolorization of azo dyes [J].

Murugesan, Kumarasamy ;

Arulmani, Manavalan ;

Nam, In-Hyun ;

Kim, Young-Mo ;

Chang, Yoon-Seok ;

Kalaichelvan, P. Thangavelu .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 72 (05) :939-946

[26] Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa [J].

Nachiyar, CV ;

Rajakumar, GS .

ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (04) :503-509

[27] Bacterial decolorization and degradation of azo dyes [J].

Pandey, Anjah ;

Singh, Poonam ;

Iyengar, Leela .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2007, 59 (02) :73-84

[28]

Parshetti G, 2006, ACTA CHIM SLOV, V53, P492

[29] INFLUENCE OF AROMATIC-SUBSTITUTION PATTERNS ON AZO DYE DEGRADABILITY BY STREPTOMYCES SPP AND PHANEROCHAETE-CHRYSOSPORIUM [J].

PASTIGRIGSBY, MB ;

PASZCZYNSKI, A ;

GOSZCZYNSKI, S ;

CRAWFORD, DL ;

CRAWFORD, RL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (11) :3605-3613

[30] Biodegradation of Reactive Blue 59 by isolated bacterial consortium PMB11 [J].

Patil, P. S. ;

Shedbalkar, U. U. ;

Kalyani, D. C. ;

Jadhav, J. P. .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 35 (10) :1181-1190

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