Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

被引：30

作者：

Cubillos-Zapata, Carolina ^{[1
,3
]}

Angulo, Ivan ^{[1
]}

Almanza, Horacio ^{[1
,4
]}

Borrego, Belen ^{[1
]}

Zamora-Ceballos, Maria ^{[1
]}

Caston, Jose R. ^{[2
]}

Mena, Ignacio ^{[1
,5
]}

Blanco, Esther ^{[1
]}

Barcena, Juan ^{[1
]}

机构：

[1] INIA CISA, Ctr Invest Sanidad Anim, Madrid, Spain

[2] CSIC, Ctr Nacl Biotecnol, Dept Struct Macromol, Madrid, Spain

[3] La Paz Hosp, IdiPAZ Inst Hlth Res, Innate Immun Grp, Madrid 28046, Spain

[4] Univ Autonoma Baja California, Fac Med & Psicol, Tijuana, Mexico

[5] Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA

来源：

VETERINARY RESEARCH | 2020年 / 51卷 / 01期

关键词：

MOLECULAR SWITCH; PROTEIN; CONTAINS; SITES;

D O I：

10.1186/s13567-020-00785-x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5 ' HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.

引用

页数：8

共 30 条

[1] Self-Assembly of the Recombinant Capsid Protein of a Swine Norovirus into Virus-Like Particles and Evaluation of Monoclonal Antibodies Cross-Reactive with a Human Strain from Genogroup II [J].

Almanza, Horacio ;

Cubillos, Carolina ;

Angulo, Ivan ;

Mateos, Francisco ;

Caston, Jose R. ;

van der Poel, Wim H. M. ;

Vinje, Jan ;

Barcena, Juan ;

Mena, Ignacio .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (12) :3971-3979

[2] The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid [J].

Bárcena, J ;

Verdaguer, N ;

Roca, R ;

Morales, M ;

Angulo, I ;

Risco, C ;

Carrascosa, JL ;

Torres, JM ;

Castón, JR .

VIROLOGY, 2004, 322 (01) :118-134

[3]

Barcena Juan, 2013, Subcell Biochem, V68, P631, DOI 10.1007/978-94-007-6552-8_21

[4] Comparative analysis of rabbit hemorrhagic disease virus (RHDV) and new RHDV2 virus antigenicity, using specific virus-like particles [J].

Barcena, Juan ;

Guerra, Beatriz ;

Angulo, Ivan ;

Gonzalez, Julia ;

Valcarcel, Felix ;

Mata, Carlos P. ;

Caston, Jose R. ;

Blanco, Esther ;

Alejo, Ali .

VETERINARY RESEARCH, 2015, 46

[5] The Cryo-Electron Microscopy Structure of Feline Calicivirus Bound to Junctional Adhesion Molecule A at 9-Angstrom Resolution Reveals Receptor-Induced Flexibility and Two Distinct Conformational Changes in the Capsid Protein VP1 [J].

Bhella, David ;

Goodfellow, Ian G. .

JOURNAL OF VIROLOGY, 2011, 85 (21) :11381-11390

[6] Multiple Correspondence Analysis on Amino Acid Properties within the Variable Region of the Capsid Protein Shows Differences between Classical and Virulent Systemic Feline Calicivirus Strains [J].

Brunet, Sylvie ;

Sigoillot-Claude, Cecile ;

Pialot, Daniel ;

Poulet, Herve .

VIRUSES-BASEL, 2019, 11 (12)

[7] Calicivirus VP2 forms a portal-like assembly following receptor engagement [J].

Conley, Michaela J. ;

McElwee, Marion ;

Azmi, Liyana ;

Gabrielsen, Mads ;

Byron, Olwyn ;

Goodfellow, Ian G. ;

Bhella, David .

NATURE, 2019, 565 (7739) :377-+

[8] Evolutionary mechanisms of persistence and diversification of a calicivirus within endemically infected natural host populations [J].

Coyne, Karen P. ;

Gaskell, Rosalind M. ;

Dawson, Susan ;

Porter, Carol J. ;

Radford, Alan D. .

JOURNAL OF VIROLOGY, 2007, 81 (04) :1961-1971

[9] Mapping neutralizing and non-neutralizing epitopes on the capsid protein of feline calicivirus [J].

Geissler, K ;

Schneider, K ;

Truyen, U .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2002, 49 (01) :55-60

[10]

Klasse P J, 2014, Adv Biol, V2014

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