Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence

被引：7
|
作者：
Rauser, Valerie [1 ]
Weinhold, Elmar [1 ]
机构：
[1] Rhein Westfal TH Aachen, Inst Organ Chem, Landoltweg 1, Aachen 52074, Germany
来源：
CHEMBIOCHEM | 2020年 / 21卷 / 17期
关键词：
DNA; G-quadruplexes; monomer; Pu27; size-exclusion chromatography; RELEVANT G-QUADRUPLEX; ELEMENT; REGION; GENE; STABILITY; INSIGHTS; BINDING;
D O I：
10.1002/cbic.202000159
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
G-Quadruplex (G4)-forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c-myc promoter into the desired monomeric G4 by alkaline treatment and refolding.
引用
收藏
页码：2445 / 2448
页数：4
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