Pyridine Inhibitor Binding to the 4Fe-4S Protein A. aeolicus IspH (LytB): A HYSCORE Investigation

被引：35

作者：

Wang, Weixue ^{[1
]}

Li, Jikun ^{[1
]}

Wang, Ke ^{[2
]}

Smirnova, Tatyana I. ^{[3
]}

Oldfield, Eric ^{[1
,2
]}

机构：

[1] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[3] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 17期

基金：

美国国家科学基金会;

关键词：

METHYLERYTHRITOL PHOSPHATE-PATHWAY; ISOPRENOID BIOSYNTHESIS; DIPHOSPHATE REDUCTASE; 2FE-2S CLUSTER; PULSE EPR; GCPE; SPECTROSCOPY; COMPLEXES; MECHANISM; HISTIDINE;

D O I：

10.1021/ja2008455

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using hyperfine sublevel correlation (HYSCORE) spectroscopy, we show that pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, opening up new routes to inhibitor design, of interest in the context of both anti-bacterial as well as anti-malarial drug discovery.

引用

页码：6525 / 6528

页数：4

共 31 条

[1] LytB protein catalyzes the terminal step of the 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis
Altincicek, B
Duin, EC
Reichenberg, A
Hedderich, R
Kollas, AK
Hintz, M
Wagner, S
Wiesner, J
Beck, E
Jomaa, H
[J]. FEBS LETTERS, 2002, 532 (03) : 437 - 440
[2] N-14 NUCLEAR-QUADRUPOLE RESONANCE-SPECTRA OF COORDINATED IMIDAZOLE
ASHBY, CIH
CHENG, CP
BROWN, TL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (19) : 6057 - 6063
[3] Van der Waals radii of elements
Batsanov, SS
[J]. INORGANIC MATERIALS, 2001, 37 (09) : 871 - 885
[4] ELECTRON-SPIN ECHO ENVELOPE MODULATION SPECTROSCOPY SUPPORTS THE SUGGESTED COORDINATION OF 2 HISTIDINE LIGANDS TO THE RIESKE FE-S CENTERS OF THE CYTOCHROME-B6F COMPLEX OF SPINACH AND THE CYTOCHROME-BC1 COMPLEXES OF RHODOSPIRILLUM-RUBRUM, RHODOBACTER-SPHAEROIDES R-26, AND BOVINE HEART-MITOCHONDRIA
BRITT, RD
SAUER, K
KLEIN, MP
KNAFF, DB
KRIAUCIUNAS, A
YU, CA
YU, L
MALKIN, R
[J]. BIOCHEMISTRY, 1991, 30 (07) : 1892 - 1901
[5] Binding of Histidine in the (Cys)3(His)1-Coordinated [2Fe-2S] Cluster of Human mitoNEET
Dicus, Michelle M.
Conlan, Andrea
Nechushtai, Rachel
Jennings, Patricia A.
Paddock, Mark L.
Britt, R. David
Stoll, Stefan
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (06) : 2037 - 2049
[6] A comparative, two-dimensional 14N ESEEM characterization of reduced [2Fe-2S] clusters in hyperthermophilic archaeal high- and low-potential Rieske-type proteins
Dikanov, SA
Shubin, AA
Kounosu, A
Iwasaki, T
Samoilova, RI
[J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2004, 9 (06): : 753 - 767
[7] A multi-frequency pulse EPR and ENDOR approach to study strongly coupled nuclei in frozen solutions of high-spin ferric heme proteins
Fittipaldi, M.
Garcia-Rubio, I.
Trandafir, F.
Gromov, I.
Schweiger, A.
Bouwen, A.
Van Doorslaer, S.
[J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (12) : 3859 - 3870
[8] Frisch M. J., 2016, Gaussian 03 Revision B.03
[9] Probing the reaction mechanism of IspH protein by x-ray structure analysis
Graewert, Tobias
Span, Ingrid
Eisenreich, Wolfgang
Rohdich, Felix
Eppinger, Joerg
Bacher, Adelbert
Groll, Michael
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (03) : 1077 - 1081
[10] ELECTRON NUCLEAR DOUBLE-RESONANCE SPECTROSCOPY OF N-15-ENRICHED PHTHALATE DIOXYGENASE FROM PSEUDOMONAS-CEPACIA PROVES THAT 2 HISTIDINES ARE COORDINATED TO THE [2FE-2S] RIESKE-TYPE CLUSTERS
GURBIEL, RJ
BATIE, CJ
SIVARAJA, M
TRUE, AE
FEE, JA
HOFFMAN, BM
BALLOU, DP
[J]. BIOCHEMISTRY, 1989, 28 (11) : 4861 - 4871

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