The Relevance of miRNA-21 in HSV-Induced Inflammation in a Mouse Model

被引：25

作者：

Choi, Bunsoon ^{[1
]}

Kim, Hyoun-Ah ^{[2
]}

Suh, Chang-Hee ^{[2
]}

Byun, Hae Ok ^{[1
]}

Jung, Ju-Yang ^{[2
]}

Sohn, Seonghyang ^{[1
,3
]}

机构：

[1] Ajou Univ, Sch Med, Dept Microbiol, Suwon 443380, South Korea

[2] Ajou Univ, Sch Med, Dept Rheumatol, Suwon 443380, South Korea

[3] Ajou Univ, Grad Sch, Dept Biomed Sci, Suwon 443380, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

microRNA-21; herpes simplex virus-induced inflammation; mouse model; Behcet's Disease; miR-21; antagomir; HERPES-SIMPLEX-VIRUS; KOYANAGI-HARADA SYNDROME; GENOME-WIDE ASSOCIATION; OCULAR BEHCETS-DISEASE; TOLL-LIKE RECEPTORS; IMMUNE-RESPONSE; T-CELLS; ABERRANT EXPRESSION; MICRORNA EXPRESSION; TH1; POLARIZATION;

D O I：

10.3390/ijms16047413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to clarify the correlation between microRNA-21 (miR-21) expression and inflammation in a herpes simplex virus (HSV)-induced Behcet's Disease (BD) mouse model. miR-21 was compared between BD patients and healthy controls in peripheral blood mononuclear cells (PBMC). For miR-21 inhibition, miR-21 antagomir was applied to BD mice. The change of symptoms was monitored. The levels of cytokines and related molecules were determined by ELISA and real time qPCR. Treatment with colchicine or pentoxifylline down-regulated the level of miR-21 with improved symptoms in mice. miR-21 inhibition was accompanied by down-regulated serum levels of IL-17 and IL-6. The expression levels of PDCD4, RhoB, PD-1, IL-12p35, and toll-like receptor-4 were also regulated by miR-21 inhibition. miR-21 was correlated with HSV-induced BD-like inflammation in mice and BD patients. The expression of miR-21 was regulated by antagomir in mice.

引用

页码：7413 / 7427

页数：15

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