Paraoxonase-1 and linoleic acid oxidation in. familial hypercholesterolemia

被引：14

作者：

van Himbergen, TM ^{[1
]}

van Tits, LJH

Hectors, MPC

de Graaf, J

Roest, M

Stalenhoef, AFH

机构：

[1] UMC Utrecht, Res Lab, Dept Clin Chem, Utrecht, Netherlands

[2] Radboud Univ Nijmegen, Ctr Med, Div Gen Internal Med, Dept Med, Nijmegen, Netherlands

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 03期

关键词：

paraoxonase-1; linoleic acid; oxidation; familial hypercholesterolemia;

D O I：

10.1016/j.bbrc.2005.05.176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serum paraoxonase-1 (PON1) is a high-density lipoprotein-associated enzyme that can inhibit low-density lipoprotein (LDL) oxidation in vitro. The role of PON1 in vivo still remains to be clarified. We investigated the effect of PON1 genotype (-107C > T and 192Q > R), concentration, paraoxonase activity, and arylesterase activity on the early phase of lipid peroxidation in plasma samples of 110 patients with heterozygous familial hypercholesterolemia. The degree of lipid oxidation was assessed by quantitation of oxidized-linoleic acid (the most abundant fatty acid present in LDL) using high performance liquid chromatography. We found a significant inverse correlation between paraoxonase activity and the oxidized-linoleic acid concentration (r = -0.22, P = 0.03), independent of baseline linoleic acid levels. These findings support an anti-oxidative role for PON1 in patients with FH, and thus may give insight into the functioning of PON1 in vivo. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：787 / 793

页数：7

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