Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

被引:30
作者
Parreiras, Patricia Martins [2 ]
Andrade, Giovanna Ivo [3 ]
do Nascimento, Telma de Figueiredo [1 ]
Oelemann, Maranibia Cardoso [1 ]
Gomes, Harrison Magdinier [1 ]
de Alencar, Andrea Padilha [5 ]
de Assis, Ronnie Antunes [5 ]
Pinto Coelho Mota, Pedro Moacyr [5 ]
da Silva Pereira, Marcia Aparecida [1 ]
Faria Lobato, Francisco Carlos [4 ]
Lage, Andrey Pereira [3 ]
Suffys, Philip Noel [1 ]
机构
[1] Inst Oswaldo Cruz Fiocruz, Lab Biol Mol Aplicada Micobacterias, BR-21040360 Rio De Janeiro, RJ, Brazil
[2] Inst Rene Rachou Fiocruz, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Lab Bacteriol Aplicada, Belo Horizonte, MG, Brazil
[4] Univ Fed Minas Gerais, Lab Bacterioses & Pesquisa, Dept Vet Prevent Med, Escola Vet, Belo Horizonte, MG, Brazil
[5] Lab Nacl Agropecuario Minas Gerais, Pedro Leopoldo, MG, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2012年 / 107卷 / 01期
关键词
Mycobacterium bovis; spoligotyping; MIRU-VNTR typing; bovine tuberculosis; molecular epidemiology; HIGHLY REPRODUCIBLE TECHNIQUES; FRAGMENT-LENGTH-POLYMORPHISM; SYSTEMATIC BACTERIOLOGY; MOLECULAR EPIDEMIOLOGY; GENUS MYCOBACTERIUM; GENETIC DIVERSITY; TUBERCULOSIS; STRAINS; COMPLEX; DNA;
D O I
10.1590/S0074-02762012000100009
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.
引用
收藏
页码:64 / 73
页数:10
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