rBTI extends Caenorhabditis elegans lifespan by mimicking calorie restriction

被引：22
作者：
Li, Jiao [1 ]
Cui, Xiaodong [1 ]
Wang, Zhuanhua [1 ]
Li, Yuying [1 ]
机构：
[1] Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Taiyuan 030006, Peoples R China
来源：
EXPERIMENTAL GERONTOLOGY | 2015年 / 67卷
关键词：
rBTI; CR; Lifespan; ROS; Insulin/IGF-1; signaling; C-ELEGANS; DIETARY RESTRICTION; OXIDATIVE STRESS; CELL-PROLIFERATION; INDUCED LONGEVITY; INSULIN; INHIBITOR; GENE; MITOHORMESIS; DAF-16/FOXO;
D O I：
10.1016/j.exger.2015.05.001
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Buckwheat trypsin inhibitor (BTI) is a low molecular weight polypeptide extracted from buckwheat. This study examined the effects of BTI on the lifespan of Caenorhabditis elegans (Caenorhabditis elegans) and investigated the mechanism involved. Our results showed that recombinant BTI (rBTI) extended life expectancy by mimicking calorie restriction (CR) in Caenorhabditis elegans. rBTI promoted formation of reactive oxygen species (ROS) via increasing respiration, induced activities of ROS defense enzymes by activating DAF- 16, and increased oxidative stress resistance and survival rates. The inhibition of ROS signal by antioxidants reduced rBTI-mediated longevity by up to 65%. Moreover, it was shown that the disruption of daf-2 abolished the extension of the lifespan and the increased ROS. Taken together, these data indicate that rBTI-mediated longevity mimics CR by down-regulating insulin/IGF-1 signaling (IIS) pathway, implying that BTI has the potential to be a novel anti-aging drug. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：62 / 71
页数：10
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