Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin

被引：44

作者：

Stirm, Laura ^{[1
,2
]}

Huypens, Peter ^{[2
,3
]}

Sass, Steffen ^{[2
,4
]}

Batra, Richa ^{[4
,5
]}

Fritsche, Louise ^{[1
,2
]}

Brucker, Sara ^{[6
]}

Abele, Harald ^{[6
]}

Hennige, Anita M. ^{[1
,7
]}

Theis, Fabian ^{[2
,4
]}

Beckers, Johannes ^{[2
,3
,8
]}

de Angelis, Martin Hrabe ^{[2
,3
,8
]}

Fritsche, Andreas ^{[1
,2
,7
]}

Haering, Hans-Ulrich ^{[1
,2
,7
,9
]}

Staiger, Harald ^{[1
,2
,3
,9
,10
]}

机构：

[1] Eberhard Karls Univ Tubingen, Helmholtz Zentrum Munchen, Inst Diabet Res & Metab Dis, Tubingen, Germany

[2] German Ctr Diabet Res DZD, Neuherberg, Germany

[3] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Expt Genet, Neuherberg, Germany

[4] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Computat Biol, Neuherberg, Germany

[5] Tech Univ Munich, Dept Dermatol & Allergy, Munich, Germany

[6] Univ Hosp Tubingen, Dept Obstet & Gynaecol, Tubingen, Germany

[7] Univ Hosp Tubingen, Dept Internal Med, Div Endocrinol Diabetol Angiol Nephrol & Clin Che, Tubingen, Germany

[8] Tech Univ Munich, Chair Expt Genet, Neuherberg, Germany

[9] Eberhard Karls Univ Tubingen, Interfac Ctr Pharmacogen & Pharma Res, Tubingen, Germany

[10] Eberhard Karls Univ Tubingen, Inst Pharmaceut Sci, Dept Pharm & Biochem, Tubingen, Germany

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

CIRCULATING MICRORNAS; EXPRESSION; METABOLISM; OBESITY; WOMEN; SENSITIVITY; ADIPOKINES; PREGNANCY; MELLITUS; GROWTH;

D O I：

10.1038/s41598-018-19200-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The number of pregnancies complicated by gestational diabetes (GDM) is increasing worldwide. To identify novel characteristics of GDM, we studied miRNA profiles of maternal and fetal whole blood cells (WBCs) from GDM and normal glucose tolerant (NGT) pregnant women matched for body mass index and maternal age. After adjustment for maternal weight gain and pregnancy week, we identified 29 mature micro-RNAs (miRNAs) up-regulated in GDM, one of which, i.e., miRNA-340, was validated by qPCR. mRNA and protein expression of PAIP1, a miRNA-340 target gene, was found down-regulated in GDM women, accordingly. In lymphocytes derived from the mothers' blood and treated in vitro, insulin increased and glucose reduced miRNA-340 expression. In fetal cord blood samples, no associations of miRNA-340 with maternal GDM were observed. Our results provide evidence for insulin-induced epigenetic, i.e., miRNA-dependent, programming of maternal WBCs in GDM.

引用

页数：12

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