Laminar flow cells for single-molecule studies of DNA-protein interactions

被引：76
作者：
Brewer, Laurence R. [1 ]
Bianco, Piero R. [2 ,3 ]
机构：
[1] Washington State Univ, Sch Chem & Bioengn, Ctr Reprod Biol, Pullman, WA 99164 USA
[2] SUNY Buffalo, Ctr Singel Mol Biophys, Dept Microbiol & Immunol, Buffalo, NY 14214 USA
[3] SUNY Buffalo, Ctr Singel Mol Biophys, Dept Biochem, Buffalo, NY 14214 USA
来源：
NATURE METHODS | 2008年 / 5卷 / 06期
关键词：
D O I：
10.1038/nmeth.1217
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Microfluidic flow cells are used in single-molecule experiments, enabling measurements to be made with high spatial and temporal resolution. We discuss the fundamental processes affecting flow cell operation and describe the flow cells in use at present for studying the interaction of optically trapped or mechanically isolated, single DNA molecules with proteins. To assist the experimentalist in flow cell selection, we review the construction techniques and materials used to fabricate both single-and multiple-channel flow cells and the advantages of each design for different experiments.
引用
收藏
页码：517 / 525
页数：9
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