Cumulated Ca2+ spike duration underlies Ca2+ oscillation frequency-regulated NFκB transcriptional activity

被引：42

作者：

Zhu, Liping ^{[1
,2
]}

Song, Shanshan ^{[1
,2
]}

Pi, Yubo ^{[1
,2
]}

Yu, Yang ^{[1
,2
]}

She, Weibin ^{[1
,2
]}

Ye, Hong ^{[1
,2
]}

Su, Yuan ^{[2
,3
]}

Hu, Qinghua ^{[1
,2
,4
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Pathophysiol, Tongji Med Coll, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Key Lab Pulm Dis, Minist Hlth China, Wuhan 430030, Peoples R China

[3] Huazhong Univ Sci & Technol, Dept Resp Med, Tongji Med Coll, Union Hosp, Wuhan 430030, Peoples R China

[4] Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Sch Publ Hlth, Tongji Med Coll, Wuhan 430030, Peoples R China

来源：

JOURNAL OF CELL SCIENCE | 2011年 / 124卷 / 15期

基金：

中国国家自然科学基金;

关键词：

Ca2+](i) oscillations; Frequency; Spike duration; Transcription; PROTEIN-KINASE-C; BRONCHIAL EPITHELIAL-CELLS; MICROVASCULAR ENDOTHELIAL-CELLS; CALCIUM OSCILLATIONS; GENE-EXPRESSION; PHORBOL ESTER; HETEROLOGOUS DESENSITIZATION; DIFFERENTIAL ACTIVATION; CHROMAFFIN CELLS; NITRIC-OXIDE;

D O I：

10.1242/jcs.082727

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

[Ca2+](i) oscillations drive downstream events, like transcription, in a frequency-dependent manner. Why [Ca2+](i) oscillation frequency regulates transcription has not been clearly revealed. A variation in [Ca2+](i) oscillation frequency apparently leads to a variation in the time duration of cumulated [Ca2+](i) elevations or cumulated [Ca2+](i) spike duration. By manipulating [Ca2+](i) spike duration, we generated a series of [Ca2+](i) oscillations with the same frequency but different cumulated [Ca2+](i) spike durations, as well as [Ca2+](i) oscillations with the different frequencies but the same cumulated [Ca2+](i) spike duration. Molecular assays demonstrated that, when generated in 'artificial' models alone, under physiologically simulated conditions or repetitive pulses of agonist exposure, [Ca2+](i) oscillation regulates NF kappa B transcriptional activity, phosphorylation of I kappa B alpha and Ca2+-dependent gene expression all in a way actually dependent on cumulated [Ca2+](i) spike duration whether or not frequency varies. This study underlines that [Ca2+](i) oscillation frequency regulates NF kappa B transcriptional activity through cumulated [Ca2+](i) spike-duration-mediated I kappa B alpha phosphorylation.

引用

页码：2591 / 2601

页数：11

共 57 条

[1] Human airway epithelial cells express interleukin-2 in vitro [J].