A genetically encoded Forster resonance energy transfer sensor for monitoring in vivo trehalose-6-phosphate dynamics

被引：21

作者：

Peroza, Estevao A. ^{[1
]}

Ewald, Jennifer C. ^{[2
]}

Parakkal, Geetha ^{[1
]}

Skotheim, Jan M. ^{[2
]}

Zamboni, Nicola ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

[2] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 474卷

关键词：

Trehalose-6-phosphate; FRET sensor; Glycolysis regulation; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; TREHALOSE; 6-PHOSPHATE; GENE; GLYCOLYSIS; FAMILY; EXPRESSION; REGULATOR; TRANSPORT; SYNTHASE;

D O I：

10.1016/j.ab.2014.12.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Trehalose-6-phosphate is a pivotal regulator of sugar metabolism, growth, and osmotic equilibrium in bacteria, yeasts, and plants. To directly visualize the intracellular levels of intracellular trehalose-6-phosphate, we developed a series of specific Forster resonance energy transfer (FRET) sensors for in vivo microscopy. We demonstrated real-time monitoring of regulation in the trehalose pathway of Escherichia con. In Saccharomyces cerevisiae, we could show that the concentration of free trehalose-6-phosphate during growth on glucose is in a range sufficient for inhibition of hexokinase. These findings support the hypothesis of trehalose-6-phosphate as the effector of a negative feedback system, similar to the inhibition of hexokinase by glucose-6-phosphate in mammalian cells and controlling glycolytic flux. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：1 / 7

页数：7