Detecting DNA methylation through changes in transverse proton relaxation

被引：7

作者：

Jaganathan, Hamsa ^{[1
]}

Wang, Pengfei ^{[1
]}

Klein, Jonathon ^{[2
]}

Lossie, Amy C. ^{[2
]}

Ivanisevic, Albena ^{[1
,3
]}

机构：

[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA

[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ANALYST | 2011年 / 136卷 / 12期

关键词：

CIRCULAR-DICHROISM; MECHANISM;

D O I：

10.1039/c1an15146k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a facile, simple method to detect DNA methylation by measuring the transverse proton relaxation behaviour. Positively charged nanoparticles are arranged along the negatively charged backbone of DNA strands through electrostatic interactions. The arrangement of NPs along DNA strands aids to amplify and compare the transverse proton relaxation signal for un-cut versus cut DNA strands cleaved by sequence specific restriction enzymes. Results from this study suggest that the presence of methylation on DNA can be detected using superparamagnetic NPs using NMR.

引用

页码：2441 / 2445

页数：5

共 35 条

[1] Hunting for the 5th base: Techniques for analyzing DNA methylation [J].

Ammerpohl, Ole ;

Martin-Subero, Jose I. ;

Richter, Julia ;

Vater, Inga ;

Siebert, Reiner .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2009, 1790 (09) :847-862

[2]

[Anonymous], 2001, ANGEW CHEM-GER EDIT

[3] Enzymatic Incorporation of Multiple Dyes for Increased Sensitivity in QD-FRET Sensing for DNA Methylation Detection [J].

Bailey, Vasudev J. ;

Keeley, Brian P. ;

Zhang, Yi ;

Ho, Yi-Ping ;

Easwaran, Hariharan ;

Brock, Malcolm V. ;

Pelosky, Kristen L. ;

Carraway, Hetty E. ;

Baylin, Stephen B. ;

Herman, James G. ;

Wang, Tza-Huei .

CHEMBIOCHEM, 2010, 11 (01) :71-74

[4] The persistence length of double stranded DNA determined using dark field tethered particle motion [J].

Brinkers, Sanneke ;

Dietrich, Heidelinde R. C. ;

de Groote, Frederik H. ;

Young, Ian T. ;

Rieger, Bernd .

JOURNAL OF CHEMICAL PHYSICS, 2009, 130 (21)

[5] PCR-free DNA detection using a magnetic bead-supported polymeric transducer and microelectromagnetic traps [J].

Dubus, Sebastein ;

Gravel, Jean-Francois ;

Le Drogoff, Boris ;

Nobert, Philippe ;

Veres, Teodor ;

Boudreau, Denis .

ANALYTICAL CHEMISTRY, 2006, 78 (13) :4457-4464

[6] Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation [J].

Feng, Fude ;

Wang, Hongzhong ;

Han, Lingli ;

Wang, Shu .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (34) :11338-11343

[7] An essential role for DNA adenine methylation in bacterial virulence [J].

Heithoff, DM ;

Sinsheimer, RL ;

Low, DA ;

Mahan, MJ .

SCIENCE, 1999, 284 (5416) :967-970

[8] Nonenzymatic detection of bacterial genomic DNA using the bio bar code assay [J].

Hill, Haley D. ;

Vega, Rafael A. ;

Mirkin, Chad A. .

ANALYTICAL CHEMISTRY, 2007, 79 (23) :9218-9223

[9] Circular Dichroism Study of the Mechanism of Formation of DNA Templated Nanowires [J].

Jaganathan, Hamsa ;

Kinsella, Joseph M. ;

Ivanisevic, Albena .

CHEMPHYSCHEM, 2008, 9 (15) :2203-2206

[10] Mechanism of Proton Relaxation for Enzyme-Manipulated, Multicomponent Gold-Magnetic Nanoparticle Chains [J].

Jaganathan, Hamsa ;

Gieseck, Richard L. ;

Hudson, Katherine ;

Kellogg, Michael ;

Ramaswamy, Aneesh K. ;

Raver, Kimberly E. ;

Smith, Tyler ;

Vacchiano, Ashley N. ;

Wager, Andrew ;

Ivanisevic, Albena .

CHEMPHYSCHEM, 2010, 11 (17) :3664-3672

← 1 2 3 4 →