Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

被引：11

作者：

McConnell, G

Smith, GL

Girkin, JM

Gurney, AM

Ferguson, AI

机构：

[1] Univ Strathclyde, Ctr Biophoton, Glasgow G4 0NR, Lanark, Scotland

[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[3] Univ Strathclyde, Inst Photon, Glasgow G4 0NW, Lanark, Scotland

[4] Univ Strathclyde, Dept Physiol & Pharmacol, Glasgow G4 0NR, Lanark, Scotland

[5] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland

来源：

OPTICS LETTERS | 2003年 / 28卷 / 19期

关键词：

D O I：

10.1364/OL.28.001742

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2. (C) 2003 Optical Society of America.

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页码：1742 / 1744

页数：3

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