SSR-based DNA fingerprinting of 18 elite Indian varieties of sesame (Sesamum indicum L.)

被引：4

作者：

Bhattacharjee, Maini ^{[1
]}

Prakash, S. H. ^{[2
]}

Roy, Souvik ^{[2
]}

Soumen, Shejal ^{[2
]}

Begum, Tamina ^{[1
]}

Dasgupta, Tapash ^{[2
]}

机构：

[1] Univ Calcutta, Dept Genet & Plant Breeding, Inst Agr Sci, Kolkata 700019, India

[2] Ramakrishna Mission Vivekananda Educ & Res Inst, Sch Agr & Rural Dev, Kolkata 700103, India

来源：

NUCLEUS-INDIA | 2020年 / 63卷 / 01期

关键词：

DNA fingerprinting; SSR markers; Variety identification; Sesame; MICROSATELLITE MARKERS; GENETIC-RELATIONSHIP; DIVERSITY; IDENTIFICATION; COLLECTION; GERMPLASM; CULTIVARS;

D O I：

10.1007/s13237-019-00290-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To assess the genetic diversity and relationship between varieties eighteen elite Indian sesame varieties were analysed using thirty sesame-specific SSR markers. A total of 134 alleles with an average number of 4.5 alleles were observed with PIC value ranging from 0.39 (CUESTSSR563) to 0.87 (CUSSR29). The dendrogram was constructed using the software NTSYS Pc (Ver. 2.20). The varieties were grouped into 5 clusters. The dissimilarity coefficients between sesame varieties based on molecular data ranged from 0.466 to 0.967. GT 10 and RT 351 showed highest distance value i.e. 0.967 and the lowest distance value was estimated between the genotypes Prachi and Nirmala. The study revealed that geographic origin is not related to genetic diversity as genotypes belonging to different geographical origin grouped in the same cluster. DNA profile of varieties exhibited good polymorphism which helps to distinctly identify and characterize sesame varieties.

引用

页码：67 / 73

页数：7

共 28 条

[1] Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers [J].