Adiponectin inhibits lymphotoxin-β receptor-mediated NF-κB signaling in human umbilical vein endothelial cells

被引：14

作者：

Xu, Yizhou ^{[1
,2
,3
,4
]}

Zhang, Chu ^{[1
,2
]}

Wang, Ningfu ^{[1
,2
]}

Ling, Feng ^{[1
,2
]}

Li, Peizhang ^{[1
,2
]}

Gao, Yan ^{[1
,2
]}

Hua, Wei ^{[3
,4
]}

机构：

[1] Nanjing Med Univ, Dept Cardiol, Hangzhou Peoples Hosp 1, Hangzhou 310006, Zhejiang, Peoples R China

[2] Nanjing Med Univ, Hangzhou Hosp, Hangzhou 310006, Zhejiang, Peoples R China

[3] Chinese Acad Med Sci, Fu Wai Hosp, Cardiac Arrhythmia Ctr, Cardiovasc Inst, Beijing 100037, Peoples R China

[4] Peking Union Med Coll, Beijing 100037, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 404卷 / 04期

关键词：

Adiponectin; AdipoR1; LTBR; Yeast two-hybrid screen; NF-kappa B; GLOBULAR ADIPONECTIN; CARDIOVASCULAR-DISEASE; TRANSCRIPTION FACTOR; DEFICIENT MICE; ATHEROSCLEROSIS; PROLIFERATION; ACTIVATION; EXPRESSION;

D O I：

10.1016/j.bbrc.2010.12.110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adiponectin exerts anti-diabetic and anti-atherogenesis properties through its 2 receptors (AdipoR1 and AdipoR2). However, the signaling pathways responsible for the anti-inflammatory effects of adiponectin are largely unknown. In this study, we identified the lymphotoxin (LT)-beta receptor (LTBR) as an interacting partner of human AdipoR1 by using a yeast two-hybrid screening. The interaction between LTBR and AdipoR1 was confirmed by co-immunoprecipitation and co-localization analysis. Furthermore, adiponectin incubation inhibited lymphotoxin-induced NF-kappa B activation and the expression of adhesion molecules in human umbilical vein endothelial cells. These results indicated that AdipoR1 interacted with LTBR and mediated the inhibition of LTBR-activated NF-kappa B pathway. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：1060 / 1064

页数：5

共 26 条

[1] Adiponectin: from obesity to cardiovascular disease [J].

Antoniades, C. ;

Antonopoulos, A. S. ;

Tousoulis, D. ;

Stefanadis, C. .

OBESITY REVIEWS, 2009, 10 (03) :269-279

[2] NF-kappa B: Ten years after [J].

Baeuerle, PA ;

Baltimore, D .

CELL, 1996, 87 (01) :13-20

[3] ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism [J].

Berg, AH ;

Combs, TP ;

Scherer, PE .

TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2002, 13 (02) :84-89

[4] LYMPHOTOXIN-BETA, A NOVEL MEMBER OF THE TNF FAMILY THAT FORMS A HETEROMERIC COMPLEX WITH LYMPHOTOXIN ON THE CELL-SURFACE [J].

BROWNING, JL ;

NGAMEK, A ;

LAWTON, P ;

DEMARINIS, J ;

TIZARD, R ;

CHOW, EPC ;

HESSION, C ;

OBRINEGRECO, B ;

FOLEY, SF ;

WARE, CF .

CELL, 1993, 72 (06) :847-856

[5] T lymphocyte-endothelial cell interactions [J].

Choi, J ;

Enis, DR ;

Koh, KP ;

Shiao, SL ;

Pober, JS .

ANNUAL REVIEW OF IMMUNOLOGY, 2004, 22 :683-709

[6] TRANSCRIPTIONAL REGULATION OF ENDOTHELIAL-CELL ADHESION MOLECULES - NF-KAPPA-B AND CYTOKINE-INDUCIBLE ENHANCERS [J].

COLLINS, T ;

READ, MA ;

NEISH, AS ;

WHITLEY, MZ ;

THANOS, D ;

MANIATIS, T .

FASEB JOURNAL, 1995, 9 (10) :899-909

[7]

de Martin R, 2000, ARTERIOSCL THROM VAS, V20, pE83

[8] Adipokines and vascular disease in diabetes [J].

Goldstein B.J. ;

Scalia R. .

Current Diabetes Reports, 2007, 7 (1) :25-33

[9] Protective vascular and myocardial effects of adiponectin [J].

Goldstein, Barry J. ;

Scalia, Rosario G. ;

Ma, Xin L. .

NATURE CLINICAL PRACTICE CARDIOVASCULAR MEDICINE, 2009, 6 (01) :27-35

[10] Adiponectin: A novel adipokine linking adipocytes and vascular function [J].

Goldstein, BJ ;

Scalia, R .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (06) :2563-2568

← 1 2 3 →