Determination of tobramycin in serum using liquid chromatography-tandem mass spectrometry and comparison with a fluorescence polarisation assay

被引：59

作者：

Keevil, BG ^{[1
]}

Lockhart, SJ ^{[1
]}

Cooper, DR ^{[1
]}

机构：

[1] Wythenshawe Hosp, S Manchester Univ Hosp NHS Trust, Dept Clin Biochem, Manchester M23 9LT, Lancs, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 794卷 / 02期

关键词：

tobramycin;

D O I：

10.1016/S1570-0232(03)00492-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a tandem mass spectrometry (LC-MS-MS) method for measuring tobramycin concentrations in serum samples and have compared it with a fluorescence polarisation immunoassay. After protein precipitation with acetonitrile supernatant was injected into the LC-MS-MS system. A C-18 cartridge (4 x 2 mm) was eluted with a step gradient of 20-100% methanol containing HFBA. The retention times were, tobramycin 1.05 min and sisomycin 1.05 min. The MRM transitions were: m/z 467.8 > 163 (tobramycin) and m/z 447.8 > 160 (sisomycin). The limit of quantification was 0.15 mg/l and the assay was linear up to 50 mg/l. Assay precision was <6% within and between batch. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：329 / 335

页数：7

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