Vitrification versus slow freezing of oocytes: effects on morphologic appearance, meiotic spindle configuration, and DNA damage

被引：98

作者：

Martinez-Burgos, Monica ^{[1
]}

Herrero, Leyre ^{[1
]}

Megias, Diego ^{[2
]}

Salvanes, Ruben ^{[1
]}

Montoya, Maria C. ^{[2
]}

Cobo, Ana C. ^{[3
]}

Garcia-Velasco, Juan A. ^{[1
,4
]}

机构：

[1] IVI Madrid, Madrid 29223, Spain

[2] Natl Canc Res Ctr CNIO, Biotechnol Programme, Confocal Microscopy & Cytometry Unit, Madrid, Spain

[3] Univ Valencia, IVI Valencia, Valencia, Spain

[4] Rey Juan Carlos Univ, Madrid, Spain

来源：

FERTILITY AND STERILITY | 2011年 / 95卷 / 01期

关键词：

Vitrification; slow freezing; cellular volume; meiotic spindle; DNA fragmentation; HIGHLY EFFICIENT VITRIFICATION; CRYOTOP METHOD; CRYOPRESERVED OOCYTES; SUCROSE CONCENTRATION; MOUSE; SURVIVAL; EMBRYOS; FRESH;

D O I：

10.1016/j.fertnstert.2010.07.1089

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

To assess possible effects on subcellular organization after cryopreservation, we compared vitrified and slowly frozen oocytes in terms of their post-warm/thaw morphology, meiotic spindle configuration, and DNA integrity. DNA integrity of cryopreserved oocytes was not altered after the procedures, but vitrification was more effective than slow cooling, as shown by higher survival rate and spindle assessment despite a higher misalignament between meiotic spindle and polar body. (Fertil Steril (R) 2011; 95:374-7. (c) 2011 by American Society for Reproductive Medicine.)

引用

页码：374 / 377

页数：4

共 33 条

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