In vivo delivery of nuclear targeted drugs for lung cancer using novel synthesis and functionalization of iron oxide nanocrystals

被引:6
作者
Vellingiri, Sreevani [1 ]
Rejeeth, Chandrababu [2 ,3 ]
Varukattu, Nipun Babu [4 ]
Sharma, Alok [5 ]
Kumar, Raju Suresh [6 ]
Almansour, Abdulrahman, I [6 ]
Arumugam, Natarajan [6 ]
Afewerki, Samson [7 ,8 ]
Kannan, Soundarapandian [9 ]
机构
[1] Bharathiar Univ, Dept Zool, Prote & Mol Cell Physiol Lab, Coimbatore, Tamil Nadu, India
[2] Shanghai Jiao Tong Univ, Sch Biomed Engn, Med X Res Inst, Shanghai 200030, Peoples R China
[3] Periyar Univ, Dept Biochem, Salem 636011, Tamil Nadu, India
[4] Shantou Univ, Canc Res Ctr, Med Coll, Shantou 515041, Guangdong, Peoples R China
[5] ISF Coll Pharm, Dept Pharmacognosy, Moga 142001, Punjab, India
[6] King Saud Univ, Coll Sci, Dept Chem, POB 2455, Riyadh 11451, Saudi Arabia
[7] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Boston, MA 02115 USA
[8] Harvard Univ MIT MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA
[9] Periyar Univ, Sch Life Sci, Div Canc Nanomed, Salem 636011, India
关键词
RECENT PROGRESS; NANOPARTICLES; THERAPY; CARRIER; BLOOD; CELLS;
D O I
10.1039/d1nj05867c
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Iron nanoparticles are typically made from inorganic precursors, but for the first time, we synthesized-Fe2O3-NCs from goat blood (a bio-precursor) employing the RBC lysis method (a molecular level approach). After that, gamma-Fe2O3-NPs were coated with PEG and combined with DOX to form nanocarriers (NC) for drug delivery. PEG-DOX-gamma-Fe2O3-NCs were physicochemically investigated using A549 lung cancer cells as a model for in vitro and in vivo studies. The result revealed that PEG-DOX-gamma-Fe2O3-NCs were 60-70 nm in size, confirming the presence of PEG and DOX in gamma-Fe2O3-NCs. Cytotoxicity, morphological abnormalities, and intracellular iron recognition were observed in a dose-dependent manner in the cell line investigation. The presence of robust DOX signals in the nucleus shows that PEG-DOX-gamma-Fe2O3-NCs were used for nuclear chemotherapy and cell uptake. In vivo, the rapid release of PEG-DOX-gamma-Fe2O3-NCs was also observed. DOX suppresses carcinogenesis and has no toxicity in the healthy organs of tumor-bearing mice, according to investigated histological study. As a result, PEG-DOX-gamma-Fe2O3-NCs synthesized from natural sources will be preferable to commercially available synthetic sources and can be employed as an anticancer agent. This nanocarrier will be developed in the future with various drug carrier systems for targeted nuclear treatment of cancer and other related diseases.
引用
收藏
页码:12488 / 12499
页数:12
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