Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction

被引：24

作者：

El-Sagheer, Afaf H. ^{[1
,2
]}

Cheong, Vee Vee ^{[3
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Suez Canal Univ, Fac Petr & Min Engn, Dept Sci & Math, Chem Branch, Suez, Egypt

[3] Nanyang Technol Univ, Sch Phys & Math Sci, Div Chem & Biol Chem, Singapore 637371, Singapore

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2011年 / 9卷 / 01期

关键词：

CLICK CHEMISTRY; CROSS-LINKING; DNA; CYCLOADDITION; PEPTIDE; PROBES; SERRS;

D O I：

10.1039/c0ob00451k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.

引用

页码：232 / 235

页数：4

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