Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction

被引：24
作者：
El-Sagheer, Afaf H. [1 ,2 ]
Cheong, Vee Vee [3 ]
Brown, Tom [1 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Suez Canal Univ, Fac Petr & Min Engn, Dept Sci & Math, Chem Branch, Suez, Egypt
[3] Nanyang Technol Univ, Sch Phys & Math Sci, Div Chem & Biol Chem, Singapore 637371, Singapore
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2011年 / 9卷 / 01期
关键词：
CLICK CHEMISTRY; CROSS-LINKING; DNA; CYCLOADDITION; PEPTIDE; PROBES; SERRS;
D O I：
10.1039/c0ob00451k
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.
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收藏
页码：232 / 235
页数：4
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