Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells

被引：69

作者：

Babbey, Clifford M. ^{[1
]}

Bacallao, Robert L. ^{[1
]}

Dunn, Kenneth W. ^{[1
]}

机构：

[1] Indiana Univ, Med Ctr, Dept Med, Div Nephrol, Indianapolis, IN 46202 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | 2010年 / 299卷 / 03期

基金：

美国国家卫生研究院;

关键词：

primary cilium; Sec8; CANINE KIDNEY-CELLS; STIMULATED GLUT4 TRANSLOCATION; POLARIZED MEMBRANE-TRANSPORT; BASOLATERAL PLASMA-MEMBRANE; MDCK CELLS; RECYCLING ENDOSOME; PRIMARY CILIOGENESIS; COMMON ENDOSOMES; MAMMALIAN-CELLS; SEC6/8; COMPLEX;

D O I：

10.1152/ajprenal.00198.2010

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Babbey CM, Bacallao RL, Dunn KW. Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells. Am J Physiol Renal Physiol 299: F495-F506, 2010. First published June 24, 2010; doi:10.1152/ajprenal.00198.2010.-Rab10, a mammalian homolog of the yeast Sec4p protein, has previously been associated with endocytic recycling and biosynthetic membrane transport in cultured epithelia and with Glut4 translocation in adipocytes. Here, we report that Rab10 associates with primary cilia in renal epithelia in culture and in vivo. In addition, we find that Rab10 also colocalizes with exocyst proteins at the base of nascent cilia, and physically interacts with the exocyst complex, as detected with anti-Sec8 antibodies. These data suggest that membrane transport to the primary cilum may be mediated by interactions between Rab10 and an exocyst complex located at the cilium base.

引用

页码：F495 / F506

页数：12

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