Development and evaluation of a Multiplex Polymerase Chain Reaction for the detection of Salmonella species

被引：0

作者：

Thong, K. L. ^{[1
]}

Teh, C. S. J. ^{[2
]}

Chua, K. H. ^{[3
]}

机构：

[1] Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Dept Med Microbiol, Fac Med, Kuala Lumpur 50603, Malaysia

[3] Univ Malaya, Dept Biomed Sci, Fac Med, Kuala Lumpur 50603, Malaysia

来源：

TROPICAL BIOMEDICINE | 2014年 / 31卷 / 04期

关键词：

TYPHOID-FEVER; GLOBAL BURDEN; IDENTIFICATION; PCR; AMPLIFICATION; SEROTYPES; DIAGNOSIS;

D O I：

暂无

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present study aims to develop a system which consists of four pairs of primers that specifically detects Salmonella spp., Salmonella serovar Typhi and Salmonella serovar Paratyphi A with an internal amplification control. The system, when applied in Polymerase Chain Reaction (PCR) under specific conditions, reaction mixture and cycling temperatures produced four bands; 784 bp, 496 bp, 332 bp and 187 bp. The DNA band 784 bp is present in all Salmonella spp., while the bands of 496 bp and 332 bp are only present in S. Paratyphi A and S. Typhi, respectively. An internal amplification control as indicated by the 187 bp shows the system is working in optimum condition in all the tests. This multiplex PCR was evaluated on 241 bacterial cultures and 691 naturally contaminated samples. Overall, this multiplex PCR detection system provides a single step for simultaneous detection of DNAs of Salmonella spp., S. Typhi and S. Paratyphi A.

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页码：689 / 697

页数：9

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