2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo

被引：58

作者：

Ao, X. ^{[1
]}

Bright, S. A. ^{[2
]}

Taylor, N. C. ^{[2
]}

Elmes, R. B. P. ^{[1
]}

机构：

[1] Natl Univ Ireland, Maynooth Univ, Dept Chem, Maynooth, Co Kildare, Ireland

[2] Trinity Coll Dublin, TBSI, Dublin 2, Ireland

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 29期

关键词：

OFF-ON PROBE; TUMOR HYPOXIA; SELECTIVE DETECTION; CANCER-THERAPY; IMAGING AGENTS; ANTICANCER; DISEASE; TH-302;

D O I：

10.1039/c7ob01406f

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.

引用

页码：6104 / 6108

页数：5

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