In Vitro Inactivation of SARS-CoV-2 Using Gamma Radiation

被引：24

作者：

Leung, Anders ^{[1
]}

Tran, Kaylie ^{[1
]}

Audet, Jonathan ^{[1
]}

Lavineway, Sherisse ^{[1
]}

Bastien, Nathalie ^{[1
]}

Krishnan, Jay ^{[1
]}

机构：

[1] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada

来源：

APPLIED BIOSAFETY | 2020年 / 25卷 / 03期

关键词：

Covid; SARS-CoV-2; inactivation; gamma radiation; pandemic; MECHANISMS; IRRADIATION; DAMAGE; DNA;

D O I：

10.1177/1535676020934242

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is classified as a Risk Group 3 pathogen; propagative work with this live virus should be conducted in biosafety level-3 (BSL-3) laboratories. However, inactivated virus can be safely handled in BSL-2 laboratories. Gamma irradiation is one of the methods used to inactivate a variety of pathogens including viruses. Objective: To determine the radiation dose required to inactivate SARS-CoV-2 and its effect, if any, on subsequent polymerase chain reaction (PCR) assay. Methods: Aliquots of SARS-CoV-2 virus culture were subjected to increasing doses of gamma radiation to determine the proper dose required to inactivate the virus. Real-time quantitative polymerase chain reaction (RT-qPCR) data from irradiated samples was compared with that of the non-irradiated samples to assess the effect of gamma radiation on PCR assay. Results: A radiation dose of 1 Mrad was required to completely inactivate 10(6.5) TCID50/ml of SARS-CoV-2. The influence of gamma radiation on PCR sensitivity was inversely related and dose-dependent up to 0.5 Mrad with no further reduction thereafter. Conclusion: Gamma irradiation can be used as a reliable method to inactivate SARS-CoV-2 with minimal effect on subsequent PCR assay.

引用

页码：157 / 160

页数：4

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