Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization

被引：5
|
作者：
Han, Chengling [1 ]
Chen, Tingting [1 ]
Zu, Lily [1 ]
机构：
[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
来源：
CHEMICAL PHYSICS LETTERS | 2010年 / 500卷 / 4-6期
基金：
中国国家自然科学基金;
关键词：
QUANTUM-YIELD; ENHANCEMENT; RHODAMINE; DYNAMICS; EUROPIUM; ASSAY;
D O I：
10.1016/j.cplett.2010.10.028
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
A terbium chelate formed with diethylenetriaminepentaacetic acid and p-aminosalicylic acid (Tb-DTPA-pAS) was labeled on a 23-mer DNA strand and used as donor in the fluorescence resonance energy transfer (FRET) study of DNA hybridization. The change in donor fluorescence lifetime with and without DNA-bind acceptor carboxytetramethylrhodamine (TAMRA) showed high energy transfer efficiency (87%). The distance between the two ends of the 23-mer double-stranded DNA was estimated. This study provides a possibility of using the Tb-chelate as a probe in the studies of the geometry-dependent interaction between DNA strands or proteins. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：323 / 326
页数：4
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