In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves

被引：21

作者：

Castilla, V ^{[1
]}

Barquero, AA ^{[1
]}

Mersich, SE ^{[1
]}

Coto, CE ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, Virol Lab, RA-1428 Buenos Aires, DF, Argentina

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 1998年 / 10卷 / 01期

关键词：

arenaviruses; Junin virus; antiviral peptide; meliacine; membrane fusion;

D O I：

10.1016/S0924-8579(98)00011-9

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Meliacine, a peptide isolated from leaves of Melin azedarach L. inhibited the multiplication of Junin virus in Vero cells treated with the compound before infection (pre-treatment) or immediately after virus adsorption. Analysis of early events following infection demonstrated that meliacine blocks virus penetration by preventing the uncoating step. The addition of meliacine at different times after infection indicated that meliacine also interferes with the release of infectious particles to the extracellular medium and inhibits the low-pH-induced fusion of infected cells. Intracellular transport of viral glycoproteins to the cell membrane was not affected by meliacine, as revealed by immunofluorescence staining. Taken together, these results suggest that meliacine affects two events of the virus replicative cycle that require membrane fusion: uncoating and budding. (C) 1998 Elsevier Science B.V./International Society of Chemotherapy. All rights reserved.

引用

页码：67 / 75

页数：9

共 30 条

[21]

MARSH M, 1989, ADV VIRUS RES, V36, P107, DOI 10.1016/S0065-3527(08)60583-7

[22] RIBAVIRIN PROPHYLAXIS AND THERAPY FOR EXPERIMENTAL ARGENTINE HEMORRHAGIC-FEVER [J].