Activin A Secreted From Peripheral Nerve Fibroblasts Promotes Proliferation and Migration of Schwann Cells

被引:15
作者
Li, Yan [1 ,2 ]
Cheng, Zhenghang [2 ]
Yu, Fanhui [2 ]
Zhang, Qi [2 ]
Yu, Shu [2 ]
Ding, Fei [2 ]
He, Qianru [2 ]
机构
[1] Soochow Univ, Sch Biol & Basic Med Sci, Suzhou, Peoples R China
[2] Nantong Univ, Coinnovat Ctr Neuroregenerat, Key Lab Neuroregenerat Jiangsu, Minist Educ, Nantong, Peoples R China
来源
FRONTIERS IN MOLECULAR NEUROSCIENCE | 2022年 / 15卷
关键词
activin A; fibroblasts; Schwann cells; proliferation; migration; cytokine array; peripheral nervous system; NEURITE OUTGROWTH;
D O I
10.3389/fnmol.2022.859349
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The peripheral nervous system has remarkable regenerative capabilities. Schwann cells and fibroblasts are known to play crucial roles in these processes. In this study, we delineated the differential effects of peripheral nerve fibroblasts and cardiac fibroblasts on Schwann cells. We found that peripheral nerve fibroblasts significantly promoted Schwann cell proliferation and migration compared with cardiac fibroblasts. The cytokine array results identified 32 of 67 proteins that were considered differentially expressed in peripheral nerve fibroblasts versus cardiac fibroblasts. Among them, 25 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. Activin A, the protein with the greatest differential expression, clearly co-localized with fibroblasts in the in vivo sciatic never injury rat model. In vitro experiments proved that activin A secreted from nerve fibroblasts is the key factor responsible for boosting proliferation and migration of Schwann cells through ALK4, ALK5, and ALK7. Overall, these findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of cytokine secretion and activin A secreted from peripheral nerve fibroblasts can promote the proliferation and migration of Schwann cells.
引用
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页数:14
相关论文
共 32 条
  • [1] Activin A induces leiomyoma cell proliferation, extracellular matrix (ECM) accumulation and myofibroblastic transformation of myometrial cells via p38 MAPK
    Bao, Huiqiong
    Sin, Thomas K.
    Zhang, Guohua
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 504 (02) : 447 - 453
  • [2] Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures
    Bohlen, Christopher J.
    Bennett, F. Chris
    Tucker, Andrew F.
    Collins, Hannah Y.
    Mulinyawe, Sara B.
    Barres, Ben A.
    [J]. NEURON, 2017, 94 (04) : 759 - +
  • [3] Single Cell Transcriptome Data Analysis Defines the Heterogeneity of Peripheral Nerve Cells in Homeostasis and Regeneration
    Chen, Bing
    Banton, Matthew C.
    Singh, Lolita
    Parkinson, David B.
    Dun, Xin-peng
    [J]. FRONTIERS IN CELLULAR NEUROSCIENCE, 2021, 15
  • [4] A NF-κB-Activin A signaling axis enhances prostate cancer metastasis
    Chen, Lanpeng
    De Menna, Marta
    Groenewoud, Arwin
    Thalmann, George N.
    Kruithof-de Julio, Marianna
    Snaar-Jagalska, B. Ewa
    [J]. ONCOGENE, 2020, 39 (08) : 1634 - 1651
  • [5] Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion
    De Berdt, Pauline
    Bottemanne, Pauline
    Bianco, John
    Alhouayek, Mireille
    Diogenes, Anibal
    Llyod, Amy
    Gerardo-Nava, Jose
    Brook, Gary A.
    Miron, Veronique
    Muccioli, Giulio G.
    des Rieux, Anne
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2018, 75 (15) : 2843 - 2856
  • [6] Nerve fibroblast impact on Schwann cell behavior
    Dreesmann, Lars
    Mittnacht, Ursula
    Lietz, Martin
    Schlosshauer, Burkhard
    [J]. EUROPEAN JOURNAL OF CELL BIOLOGY, 2009, 88 (05) : 285 - 300
  • [7] Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
    He, Qianru
    Yu, Fanhui
    Li, Yan
    Sun, Junjie
    Ding, Fei
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (159):
  • [8] Activin A stimulates the proliferation and differentiation of cardiac fibroblasts via the ERK1/2 and p38-MAPK pathways
    Hu, Juan
    Wang, Xi
    Wei, Shao-Ming
    Tang, Yan-Hong
    Zhou, Qin
    Huang, Cong-Xin
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2016, 789 : 319 - 327
  • [9] Transplantation of neural scaffolds consisting of dermal fibroblast-reprogrammed neurons and 3D silk fibrous materials promotes the repair of spinal cord injury
    Hu, Ya'nan
    Zhang, Feng
    Zhong, Wentao
    Liu, Ya'nan
    He, Qisheng
    Yang, Min
    Chen, Huanhuan
    Xu, Xiaojing
    Bian, Kaipeng
    Xu, Jianwei
    Li, Jian
    Shen, Yixin
    Zhang, Huanxiang
    [J]. JOURNAL OF MATERIALS CHEMISTRY B, 2019, 7 (47) : 7525 - 7539
  • [10] Nuclear translocation of Atox1 potentiates activin A-induced cell migration and colony formation in colon cancer
    Jana, Arundhati
    Das, Archita
    Krett, Nancy L.
    Guzman, Grace
    Thomas, Alexandra
    Mancinelli, Georgina
    Bauer, Jessica
    Ushio-Fukai, Masuko
    Fukai, Tohru
    Jung, Barbara
    [J]. PLOS ONE, 2020, 15 (01):