Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

被引：34

作者：

Ahn, Min Yong ^{[1
]}

Oh, Tae Rin ^{[1
]}

Seo, Dong Hye ^{[1
]}

Kim, Jong Hum ^{[1
]}

Cho, Na Hyun ^{[1
]}

Kim, Woo Taek ^{[1
]}

机构：

[1] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Syst Biol, Seoul 03722, South Korea

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2018年 / 230卷

基金：

新加坡国家研究基金会;

关键词：

Arabidopsis group XIV E2; ERAD; Drought stress response; Interactions between E2 and E3; PUB19; UBC; ABSCISIC-ACID; E3; E2; LIGASES; COMPONENT; UBC32; MOTIF;

D O I：

10.1016/j.jplph.2018.08.010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

引用

页码：73 / 79

页数：7

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