Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain

被引：407
作者：
Li, Anan [1 ]
Gong, Hui [1 ]
Zhang, Bin [1 ]
Wang, Qingdi [1 ]
Yan, Cheng [1 ]
Wu, Jingpeng [1 ]
Liu, Qian [1 ]
Zeng, Shaoqun [1 ]
Luo, Qingming [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China
来源：
SCIENCE | 2010年 / 330卷 / 6009期
基金：
中国国家自然科学基金;
关键词：
VISUALIZATION;
D O I：
10.1126/science.1191776
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The neuroanatomical architecture is considered to be the basis for understanding brain function and dysfunction. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a three-dimensional structural data set of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other.
引用
收藏
页码：1404 / 1408
页数：5
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