DIELECTROPHORETIC (DEP) SEPARATION OF LIVE/DEAD CELLS ON A GLASS SLIDE FUNCTIONALIZED WITH INTERDIGITATED 3D SILICON RING MICROELECTRODES

被引：0

作者：

Xing, Xiaoxing ^{[1
]}

Yobas, Levent ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China

来源：

2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS) | 2014年

关键词：

ARRAY;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper describes a microfluidic device which offers a dielectrophoretic solution to cell sorting via interdigitated 3D silicon (Si) electrodes on a transparent glass substrate. The Si electrodes with a self-aligned array of lateral rings were fabricated through a single mask process on an anodically bonded Si-glass wafer. The device has been characterized for separation of live/dead human colorectal carcinoma cells at select flow rates and voltages. The live cell capture and dead cell removal efficiencies both reached 90% at an AC activation of 5V(p) 450kHz applied against a flow rate up to 0.25ml/hr. The device disclosed here, with successful demonstration of continuous live/dead cell separation, offers great potential as a bioparticle sorting front-end module for a lab-on-a chip system.

引用

页码：951 / 954

页数：4