DIELECTROPHORETIC (DEP) SEPARATION OF LIVE/DEAD CELLS ON A GLASS SLIDE FUNCTIONALIZED WITH INTERDIGITATED 3D SILICON RING MICROELECTRODES
被引:0
|
作者:
Xing, Xiaoxing
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China
Xing, Xiaoxing
[1
]
Yobas, Levent
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China
Yobas, Levent
[1
]
机构:
[1] Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China
来源:
2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)
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2014年
关键词:
ARRAY;
D O I:
暂无
中图分类号:
TM [电工技术];
TN [电子技术、通信技术];
学科分类号:
0808 ;
0809 ;
摘要:
This paper describes a microfluidic device which offers a dielectrophoretic solution to cell sorting via interdigitated 3D silicon (Si) electrodes on a transparent glass substrate. The Si electrodes with a self-aligned array of lateral rings were fabricated through a single mask process on an anodically bonded Si-glass wafer. The device has been characterized for separation of live/dead human colorectal carcinoma cells at select flow rates and voltages. The live cell capture and dead cell removal efficiencies both reached 90% at an AC activation of 5V(p) 450kHz applied against a flow rate up to 0.25ml/hr. The device disclosed here, with successful demonstration of continuous live/dead cell separation, offers great potential as a bioparticle sorting front-end module for a lab-on-a chip system.