MAPKs Are Not Involved in Triptolide-Induced Cell Growth Inhibition and Apoptosis in Prostate Cancer Cell Lines with Different p53 Status

被引：12

作者：

Li, Wei ^{[1
,2
]}

Liu, Yong ^{[1
]}

Li, Xue-Xia ^{[3
]}

Yu, Yang ^{[1
]}

Wu, Jing-Jing ^{[1
]}

Wang, Qing ^{[4
]}

Huo, Hong ^{[1
]}

Wang, Li-Ming ^{[5
]}

Yang, Ling ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

[3] Dalian Univ, Affiliated Zhongshan Hosp, Dept Oncol, Dalian, Peoples R China

[4] Dalian Univ Technol, Dalian, Peoples R China

[5] Dalian Med Univ, Affiliated Hosp 2, Dept Gen Surg, Dalian 116027, Peoples R China

来源：

PLANTA MEDICA | 2011年 / 77卷 / 01期

基金：

中国国家自然科学基金;

关键词：

triptolide; Tripterygium wilfordii; Celastraceae; prostate cancer; p53; mitogen activated protein kinases (MAPKs); PRIMARY CULTURES; ACTIVATION; KINASE; PROTEIN; P38; CASPASE-3; PATHWAYS; BCL-2; JNK; PHOSPHORYLATION;

D O I：

10.1055/s-0030-1250076

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Triptolide showed excellent antitumor activity against several solid tumors. However, its mechanism has not been fully understood. To further elucidate it, the effects of mitogen activated protein kinases (MAPKs) on the activity of triptolide towards prostate cancer cell lines were investigated in the present study using both LNCaP (p53 positive and androgen-dependent) and PC-3 (p53 deficient and androgen-independent) cells. Our results showed that triptolide exerted potent growth inhibitory and apoptotic effects on both cell lines, and the effects were independent of the expression of p53. Although upregulation of ERK and JNK phosphorylation was observed after the triptolide treatment, the results with inhibitors showed that these MAPKs were not involved in the mechanism of triptolide activity in human prostate cancer cell lines with different p53 status.

引用

页码：27 / 31

页数：5

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