Clearing and Labeling Techniques for Large-Scale Biological Tissues

被引：86

作者：

Seo, Jinyoung ^{[1
]}

Choe, Minjin ^{[2
]}

Kim, Sung-Yon ^{[1
,2
,3
,4
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Seoul 08826, South Korea

[2] Seoul Natl Univ, Dept Biophys & Chem Biol, Seoul 08826, South Korea

[3] Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea

[4] Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 08826, South Korea

来源：

MOLECULES AND CELLS | 2016年 / 39卷 / 06期

关键词：

3D volume imaging; CLARITY; large-scale tissue clearing; stochastic electrotransport; SWITCH; whole-mount labeling; LIGHT-SHEET MICROSCOPY; SINGLE-CELL RESOLUTION; WHOLE-BRAIN; INTACT-TISSUE; MOUSE-BRAIN; AGENT; RECONSTRUCTION; AMPLIFICATION; TOMOGRAPHY; EXPRESSION;

D O I：

10.14348/molcells.2016.0088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clearing and labeling techniques for large-scale biological tissues enable simultaneous extraction of molecular and structural information with minimal disassembly of the sample, facilitating the integration of molecular, cellular and systems biology across different scales. Recent years have witnessed an explosive increase in the number of such methods and their applications, reflecting heightened interest in organ-wide clearing and labeling across many fields of biology and medicine. In this review, we provide an overview and comparison of existing clearing and labeling techniques and discuss challenges and opportunities in the investigations of large-scale biological systems.

引用

页码：439 / 446

页数：8

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