Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres

被引：170

作者：

Jeffery, Nathan S. ^{[1
]}

Stephenson, Robert S. ^{[1
]}

Gallagher, James A. ^{[1
]}

Jarvis, Jonathan C. ^{[1
]}

Cox, Philip G. ^{[1
]}

机构：

[1] Univ Liverpool, Inst Ageing & Chron Dis, Dept Musculoskeletal Biol, Liverpool L69 3GE, Merseyside, England

来源：

JOURNAL OF BIOMECHANICS | 2011年 / 44卷 / 01期

基金：

英国自然环境研究理事会; 英国工程与自然科学研究理事会;

关键词：

Muscle; Myoarchitecture; MicroCT; Fascicle; Non-destructive; Pennation; Iodine; Contrast enhanced; Epimysium; Perimysium; Endomysium; DIFFUSION TENSOR; QUANTITATIVE ASSESSMENT; SKELETAL-MUSCLE; ARCHITECTURE; PARAMETERS; MORPHOLOGY; HINDLIMB; TRACKING; GLYCOGEN; COMPLEX;

D O I：

10.1016/j.jbiomech.2010.08.027

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We illustrate here microCT images in which contrast between muscle and connective tissue has been achieved by means of staining with iodine. Enhancement is shown to be dependent on the concentration of iodine solution (I2KI), time in solution and specimen size. Histological examination confirms that the arrangement of individual muscle fibres can be visualised on the enhanced microCT images, and that the iodine accumulates in the muscle fibres in preference to the surrounding connective tissues. We explore the application of this technique to describe the fibrous structure of skeletal muscle, and conclude that it has the potential to become a non-destructive and cost-effective method for investigating muscle fascicle architecture, particularly in comparative morphological studies. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：189 / 192

页数：4

共 20 条

[1]

BOCK W J, 1972, Anatomischer Anzeiger, V130, P222

[2] RELATIONSHIP BETWEEN MUSCLE-FIBER TYPES AND SIZES AND MUSCLE ARCHITECTURAL PROPERTIES IN THE MOUSE HINDLIMB [J].