Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres

被引:162
作者
Jeffery, Nathan S. [1 ]
Stephenson, Robert S. [1 ]
Gallagher, James A. [1 ]
Jarvis, Jonathan C. [1 ]
Cox, Philip G. [1 ]
机构
[1] Univ Liverpool, Inst Ageing & Chron Dis, Dept Musculoskeletal Biol, Liverpool L69 3GE, Merseyside, England
基金
英国工程与自然科学研究理事会; 英国自然环境研究理事会;
关键词
Muscle; Myoarchitecture; MicroCT; Fascicle; Non-destructive; Pennation; Iodine; Contrast enhanced; Epimysium; Perimysium; Endomysium; DIFFUSION TENSOR; QUANTITATIVE ASSESSMENT; SKELETAL-MUSCLE; ARCHITECTURE; PARAMETERS; MORPHOLOGY; HINDLIMB; TRACKING; GLYCOGEN; COMPLEX;
D O I
10.1016/j.jbiomech.2010.08.027
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We illustrate here microCT images in which contrast between muscle and connective tissue has been achieved by means of staining with iodine. Enhancement is shown to be dependent on the concentration of iodine solution (I2KI), time in solution and specimen size. Histological examination confirms that the arrangement of individual muscle fibres can be visualised on the enhanced microCT images, and that the iodine accumulates in the muscle fibres in preference to the surrounding connective tissues. We explore the application of this technique to describe the fibrous structure of skeletal muscle, and conclude that it has the potential to become a non-destructive and cost-effective method for investigating muscle fascicle architecture, particularly in comparative morphological studies. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:189 / 192
页数:4
相关论文
共 20 条
  • [1] BOCK W J, 1972, Anatomischer Anzeiger, V130, P222
  • [2] RELATIONSHIP BETWEEN MUSCLE-FIBER TYPES AND SIZES AND MUSCLE ARCHITECTURAL PROPERTIES IN THE MOUSE HINDLIMB
    BURKHOLDER, TJ
    FINGADO, B
    BARON, S
    LIEBER, RL
    [J]. JOURNAL OF MORPHOLOGY, 1994, 221 (02) : 177 - 190
  • [3] Micro-Computed Tomographical Imaging of Soft Biological Materials Using Contrast Techniques
    Faraj, Kaeuis A.
    Cuijpers, Vincent M. J. I.
    Wismans, Ronnie G.
    Walboomers, X. Frank
    Jansen, John A.
    van Kuppevelt, Toin H.
    Daamen, Willeke F.
    [J]. TISSUE ENGINEERING PART C-METHODS, 2009, 15 (03) : 493 - 499
  • [4] MUSCLE-FIBER ARCHITECTURE IN THE HUMAN LOWER-LIMB
    FRIEDERICH, JA
    BRAND, RA
    [J]. JOURNAL OF BIOMECHANICS, 1990, 23 (01) : 91 - 95
  • [5] Glycogen in the nervous system of vertebrates
    Gage, SH
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1917, 27 (04) : 451 - 465
  • [6] GAGE SH, 1906, T AM MICROSC SOC, V28, P203
  • [7] Hip joint contact force in the emu (Dromaius novaehollandiae) during normal level walking
    Goetz, Jessica E.
    Derrick, Timothy R.
    Pedersen, Douglas R.
    Robinson, Duane A.
    Conzemius, Michael G.
    Baer, Thomas E.
    Brown, Thomas D.
    [J]. JOURNAL OF BIOMECHANICS, 2008, 41 (04) : 770 - 778
  • [8] Determination of mouse skeletal muscle architecture using three-dimensional diffusion tensor imaging
    Heemskerk, AM
    Strijkers, GJ
    Vilanova, A
    Drost, MR
    Nicolay, K
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2005, 53 (06) : 1333 - 1340
  • [9] Quantitative Assessment of DTI-Based Muscle Fiber Tracking and Optimal Tracking Parameters
    Heemskerk, Anneriet M.
    Sinha, Tuhin K.
    Wilson, Kevin J.
    Ding, Zhaohua
    Damon, Bruce M.
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2009, 61 (02) : 467 - 472
  • [10] Kargo WJ, 2002, J EXP BIOL, V205, P1987