Comparative analysis of human, bovine, and murine Oct-4 upstream promoter sequences

被引：146

作者：

Nordhoff, V

Hübner, K

Bauer, A

Orlova, I

Malapetsa, A

Schöler, HR

机构：

[1] Univ Penn, Sch Vet Med, New Bolton Ctr, Ctr Anim Transgenesis & Germ Cell Res, Kennett Square, PA 19348 USA

[2] EMBL, Gene Express Programme, D-69126 Heidelberg, Germany

来源：

MAMMALIAN GENOME | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1007/s003350010279

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Oct-4 gene encodes a transcription factor that is specifically expressed in embryonic stem cells and germ cells of the mouse embryo. Cells that differentiate into somatic tissues lose Oct-4 expression. Regulation of Oct-4 gene transcription involves a TATA-less minimal promoter and two upstream elements: the proximal (PE) and distal enhancers (DE). We report here the nucleotide sequence of the 5' upstream regulatory regions of the human and murine Oct-4 genes. A comparative alignment analysis between these regions and those of the bovine Oct-4 ortholog reveals four conserved regions of homology (CR 1 to 4) between these species (66-94% conservation). The 1A sequence within the mouse PE is located approximately half-way between CR 2 and CR 3. A putative Sp1/Sp3 binding site and the overlapping hormone responsive element (HRE) in CR I are identical in all three species. A high number of CCC(A/T)CCC motifs exhibit various levels of homology in these upstream regions. We discuss the importance of these and other sequences and present candidate factors that may bind and regulate Oct-4 gene expression.

引用

页码：309 / 317

页数：9

共 54 条

[11] THE POU DOMAIN - VERSATILITY IN TRANSCRIPTIONAL REGULATION BY A FLEXIBLE 2-IN-ONE DNA-BINDING DOMAIN [J].

HERR, W ;

CLEARY, MA .

GENES & DEVELOPMENT, 1995, 9 (14) :1679-1693

[12] DNA-BINDING AND TRANSCRIPTIONAL REGULATORY ACTIVITY OF MAMMALIAN ACHAETE-SCUTE HOMOLOGOUS (MASH) PROTEINS REVEALED BY INTERACTION WITH A MUSCLE-SPECIFIC ENHANCER [J].

JOHNSON, JE ;

BIRREN, SJ ;

SAITO, T ;

ANDERSON, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3596-3600

[13] Expression pattern of Oct-4 in preimplantation embryos of different species [J].

Kirchhof, N ;

Carnwath, JW ;

Lemme, E ;

Anastassiadis, K ;

Schöler, H ;

Niemann, H .

BIOLOGY OF REPRODUCTION, 2000, 63 (06) :1698-1705

[14]

KRISHNAN BR, 1995, GENOMICS, V30, P53

[15]

LABOSKY PA, 1994, CIBA F SYMP, V182, P157

[16] MEASUREMENT OF THE BINDING OF TRANSCRIPTION FACTOR SP1 TO A SINGLE GC BOX RECOGNITION SEQUENCE [J].

LETOVSKY, J ;

DYNAN, WS .

NUCLEIC ACIDS RESEARCH, 1989, 17 (07) :2639-2653

[17]

Merchant JL, 1996, MOL CELL BIOL, V16, P6644

[18] Retinoic acid-mediated down-regulation of Oct3/4 coincides with the loss of promoter occupancy in vivo [J].

Minucci, S ;

Botquin, V ;

Yeom, YI ;

Dey, A ;

Sylvester, I ;

Zand, DJ ;

Ohbo, K ;

Ozato, K ;

Scholer, HR .

EMBO JOURNAL, 1996, 15 (04) :888-899

[19] Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4 [J].

Nichols, J ;

Zevnik, B ;

Anastassiadis, K ;

Niwa, H ;

Klewe-Nebenius, D ;

Chambers, I ;

Schöler, H ;

Smith, A .

CELL, 1998, 95 (03) :379-391

[20] Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells [J].

Niwa, H ;

Miyazaki, J ;

Smith, AG .

NATURE GENETICS, 2000, 24 (04) :372-376

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