Compound K attenuates stromal cell-derived growth factor 1 (SDF-1)-induced migration of C6 glioma cells

被引:38
作者
Kim, Hyuck [1 ]
Roh, Hyo Sun [2 ]
Kim, Jai Eun [3 ]
Park, Sun Dong [4 ]
Park, Won Hwan [1 ]
Moon, Jin-Young [2 ]
机构
[1] Dongguk Univ, Dept Diagnost, Coll Korean Med, Goyang 10326, South Korea
[2] Dongguk Univ, Dept Acupoint, Coll Korean Med, Dongguk Ro 32, Goyang 10326, South Korea
[3] Dongguk Univ, Dept Pathol, Coll Korean Med, Dongguk Ro 32, Goyang 10326, South Korea
[4] Dongguk Univ, Dept Prescript, Coll Korean Med, Dongguk Ro 32, Goyang 10326, South Korea
关键词
Panax ginseng; compound K; SDF-1; anti-tumor; C6; glioma; GINSENG SAPONIN; PANAX-GINSENG; CANCER CELLS; IN-VITRO; CHEMOKINE RECEPTOR; NERVOUS-SYSTEM; TUMOR-GROWTH; CXCR4; INHIBITION; METABOLITE;
D O I
10.4162/nrp.2016.10.3.259
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
BACKGROUND/OBJECTIVES: Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and chemokine receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential in cancer therapy. However, the inhibitory effect of CK on SDF-1 pathway-induced migration of glioma has not yet been established. MATERIALS/METHODS: Cytotoxicity of CK in C6 glioma cells was determined using an EZ-Cytox cell viability assay kit. Cell migration was tested using the wound healing and Boyden chamber assay. Phosphorylation levels of protein kinase C (PKC)alpha and extracellular signal-regulated kinase (ERK) were measured by western blot assay, and matrix metallopeptidases (MMP) were measured by gelatin-zymography analysis. RESULTS: CK significantly reduced the phosphorylation of PKC alpha and ERK1/2, expression of MMP9 and MMP2, and inhibited the migration of C6 glioma cells under SDF-1-stimulated conditions. CONCLUSIONS: CK is a cell migration inhibitor that inhibits C6 glioma cell migration by regulating its downstream signaling molecules including PKC alpha, ERK1/2, and MMPs.
引用
收藏
页码:259 / 264
页数:6
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