Long Noncoding RNAs with Enhancer-like Function in Human Cells

被引：1596

作者：

Orom, Ulf Andersson ^{[1
]}

Derrien, Thomas ^{[2
]}

Beringer, Malte ^{[1
]}

Gumireddy, Kiranmai ^{[1
]}

Gardini, Alessandro ^{[1
]}

Bussotti, Giovanni ^{[2
]}

Lai, Fan ^{[1
]}

Zytnicki, Matthias ^{[2
]}

Notredame, Cedric ^{[2
]}

Huang, Qihong ^{[1
]}

Guigo, Roderic ^{[2
]}

Shiekhattar, Ramin ^{[1
,2
,3
]}

机构：

[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

[2] UPF, Ctr Genom Regulat CRG, Barcelona, Spain

[3] ICREA, Barcelona, Spain

来源：

CELL | 2010年 / 143卷 / 01期

关键词：

TRANSCRIPTION; GENE; REVEALS; IDENTIFICATION; MECHANISMS; EXPRESSION; POSITION; ELEMENT; GENOME; REGION;

D O I：

10.1016/j.cell.2010.09.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.

引用

页码：46 / 58

页数：13

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