Identification of miR-1 as a micro RNA that supports late-stage differentiation of growth cartilage cells

被引：33

作者：

Sumiyoshi, Kumi ^{[1
,2
]}

Kubota, Satoshi ^{[1
]}

Ohgawara, Toshihiro ^{[1
,3
]}

Kawata, Kazumi ^{[1
]}

Nishida, Takashi ^{[1
]}

Shimo, Tsuyoshi ^{[3
]}

Yamashiro, Takashi ^{[2
]}

Takigawa, Masaharu ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent, Okayama 7008525, Japan

[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthodont, Okayama 7008525, Japan

[3] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral & Maxillofacial Surg & Biopathol, Okayama 7008525, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 402卷 / 02期

基金：

日本学术振兴会;

关键词：

Chondrocyte; miRNA; Hypertrophy; Gene regulation; Differentiation; HUMAN CHONDROSARCOMA; ESTABLISHMENT; CHONDROCYTES; EXPRESSION; CCN2/CTGF; DICER; GENE; LINE;

D O I：

10.1016/j.bbrc.2010.10.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The process of endochondral ossification is strictly regulated by a variety of extracellular and intracellular factors. Recently, it has become recognized that specific miRNAs are involved in this process by regulating the expression of the relevant genes at the post-transcriptional level. In this present study we obtained the first evidence of the involvement of a specific micro RNA (miRNA) in the regulation of the chondrocyte phenotype during late stages of differentiation. By use of the microarray technique, miR-1 was identified as this miRNA, the expression of which was most repressed upon hypertrophic differentiation. Transfection of human chondrocytic HCS-2/8 cells and chicken normal chondrocytes with miR-1 led to repressed expression of aggrecan, the major cartilaginous proteoglycan gene. Therefore, miR-1 was found to be involved in the regulation of the chondrocytic phenotype and thus to play an important role in chondrocytes during the late stage of the differentiation process, maintaining the integrity of the cartilage tissue. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：286 / 290

页数：5

共 23 条

[1] MicroRNA-27b Regulates the Expression of Matrix Metalloproteinase 13 in Human Osteoarthritis Chondrocytes [J].